BCM-HGSC protocols for sequencing library construction
Applied Biosystems SOLiD™ System
This protocol provides instructions for preparing DNA fragment libraries for targeted resequencing using NimbleGen Sequence Capture with the Applied Biosystems SOLiD™ System sequencing platform.
The complete TrTA-based multiplex capture sequencing protocol for SOLiD™ Platform
This protocol describes the process of utilizing AB 96 barcodes to multiplex target enrichment with NimbleGen microarrays or liquid-phase probes.
illumina
illumina Barcoded Paired-End_Capture_Library_Preparation
This protocol provides instructions for preparing DNA paired-end capture libraries for targeted sequencing by illumina platforms.
illumina Barcoded Paired-End Whole Genome Shotgun Library Preparation
This protocol provides instructions for preparing barcoded paired-end whole genome shotgun (WGS) libraries for sequencing by illumina platforms (GAII, HiSeq and MiSeq).
illumina Non-Barcoded Paired-End Capture Library Preparation
This protocol provides instructions for preparing non-barcoded paired-end capture libraries for targeted sequencing by illumina platforms.
illumina Paired-end Whole Exome Capture Library Preparation Using Full-length Index Adaptors and KAPA DNA Polymerase
This protocol provides instructions for preparing DNA paired-end capture libraries for targeted sequencing by Illumina platforms. It involves using the Covaris S2 system for DNA fragmentation, using the NEBNext End Repair, A-tailing, and Ligation Module kits for end modification, using the 2X KAPA HiFi HotStart ReadyMix for ligation-mediated PCR (LM-PCR),as well as using NimbleGen liquid probe sets for solution-based single or co-capturing target enrichment. Illumina full-length index adaptors are used in the ligation reaction.
This protocol provides instructions for preparing DNA paired-end capture libraries for targeted sequencing by Illumina platforms. It involves using the Covaris S2 system for shearing DNA samples, using the NEBNext End Repair, A-Tailing, and Ligation Modules with non-index adaptors for DNA modification, using the 2X Phusion High-Fidelity PCR Master Mix and index PCR primers for ligation-mediated PCR (LM-PCR),as well as using Nimblegen liquid probe sets for solution-based single capture target enrichment. Quality control standard or criteria for each major procedural step is also addressed here.
illumina PCR-free Indexed Paired-End Library Construction
This protocol provides instructions for manual preparation of PCR-free indexed paired-end whole genome shotgun (WGS) libraries for Illumina sequencing