Accurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0).

TitleAccurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0).
Publication TypeJournal Article
Year of Publication1995
AuthorsMetzker, ML, Allain, KM, Gibbs, RA
JournalComput Appl Biosci
Volume11
Issue2
Pagination187-94
Date Published1995 Apr
ISSN0266-7061
KeywordsAlgorithms, Bacteriophage M13, DNA, Viral, Electrophoresis, Agar Gel, Female, Genetic Carrier Screening, HIV Infections, HIV-1, Humans, Image Processing, Computer-Assisted, Microcomputers, Muscular Dystrophies, Polymerase Chain Reaction
Abstract

GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann (1.0) uses a statistical method to discriminate DNA from local background fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann (1.0)'s option box. We demonstrate that GelScann(1.0) can accurately determine DNA that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing.

Alternate JournalComput. Appl. Biosci.
PubMed ID7620992
Grant List1 P30 AI36211 / AI / NIAID NIH HHS / United States
1 P30 HG00210 / HG / NHGRI NIH HHS / United States
1 R01 HG00823 / HG / NHGRI NIH HHS / United States