Accurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0).

Title	Accurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0).
Publication Type	Journal Article
Year of Publication	1995
Authors	Metzker, ML, Allain, KM, Gibbs, RA
Journal	Comput Appl Biosci
Volume	11
Issue	2
Pagination	187-94
Date Published	1995 Apr
ISSN	0266-7061
Keywords	Algorithms, Bacteriophage M13, DNA, Viral, Electrophoresis, Agar Gel, Female, Genetic Carrier Screening, HIV Infections, HIV-1, Humans, Image Processing, Computer-Assisted, Microcomputers, Muscular Dystrophies, Polymerase Chain Reaction
Abstract	GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann (1.0) uses a statistical method to discriminate DNA from local background fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann (1.0)'s option box. We demonstrate that GelScann(1.0) can accurately determine DNA that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing.
DOI	10.1093/bioinformatics/11.2.187
Alternate Journal	Comput Appl Biosci
PubMed ID	7620992
Grant List	1 P30 AI36211 / AI / NIAID NIH HHS / United States 1 P30 HG00210 / HG / NHGRI NIH HHS / United States 1 R01 HG00823 / HG / NHGRI NIH HHS / United States