Ago HITS-CLIP expands understanding of Kaposi's sarcoma-associated herpesvirus miRNA function in primary effusion lymphomas.

TitleAgo HITS-CLIP expands understanding of Kaposi's sarcoma-associated herpesvirus miRNA function in primary effusion lymphomas.
Publication TypeJournal Article
Year of Publication2012
AuthorsHaecker, I, Gay, LA, Yang, Y, Hu, J, Morse, AM, McIntyre, LM, Renne, R
JournalPLoS Pathog
Date Published2012
KeywordsApoptosis, Cell Cycle, Cell Line, Cyclin D1, Herpesvirus 8, Human, Humans, Lymphoma, Primary Effusion, MicroRNAs, RNA, Neoplasm, RNA, Viral, Sarcoma, Kaposi

KSHV is the etiological agent of Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and a subset of multicentricCastleman's disease (MCD). The fact that KSHV-encoded miRNAs are readily detectable in all KSHV-associated tumors suggests a potential role in viral pathogenesis and tumorigenesis. MiRNA-mediated regulation of gene expression is a complex network with each miRNA having many potential targets, and to date only few KSHV miRNA targets have been experimentally determined. A detailed understanding of KSHV miRNA functions requires high-through putribonomics to globally analyze putative miRNA targets in a cell type-specific manner. We performed Ago HITS-CLIP to identify viral and cellular miRNAs and their cognate targets in two latently KSHV-infected PEL cell lines. Ago HITS-CLIP recovered 1170 and 950 cellular KSHV miRNA targets from BCBL-1 and BC-3, respectively. Importantly, enriched clusters contained KSHV miRNA seed matches in the 3'UTRs of numerous well characterized targets, among them THBS1, BACH1, and C/EBPβ. KSHV miRNA targets were strongly enriched for genes involved in multiple pathways central for KSHV biology, such as apoptosis, cell cycle regulation, lymphocyte proliferation, and immune evasion, thus further supporting a role in KSHV pathogenesis and potentially tumorigenesis. A limited number of viral transcripts were also enriched by HITS-CLIP including vIL-6 expressed only in a subset of PEL cells during latency. Interestingly, Ago HITS-CLIP revealed extremely high levels of Ago-associated KSHV miRNAs especially in BC-3 cells where more than 70% of all miRNAs are of viral origin. This suggests that in addition to seed match-specific targeting of cellular genes, KSHV miRNAs may also function by hijacking RISCs, thereby contributing to a global de-repression of cellular gene expression due to the loss of regulation by human miRNAs. In summary, we provide an extensive list of cellular and viral miRNA targets representing an important resource to decipher KSHV miRNA function.

Alternate JournalPLoS Pathog.
PubMed ID22927820
PubMed Central IDPMC3426530
Grant ListR01CA119917 / CA / NCI NIH HHS / United States
UL1 TR000064 / TR / NCATS NIH HHS / United States
R01CA88763 / CA / NCI NIH HHS / United States
RC2CA148407 / CA / NCI NIH HHS / United States
R01 CA119917 / CA / NCI NIH HHS / United States