| Title | An allylic/acyclic adenosine nucleoside triphosphate for termination of DNA synthesis by DNA template-dependent polymerases. |
| Publication Type | Journal Article |
| Year of Publication | 1999 |
| Authors | Martinez, CI, Thoresen, LH, Gibbs, RA, Burgess, K |
| Journal | Nucleic Acids Res |
| Volume | 27 |
| Issue | 5 |
| Pagination | 1271-4 |
| Date Published | 1999 Mar 01 |
| ISSN | 0305-1048 |
| Keywords | Adenosine Triphosphate, Base Sequence, Boron Compounds, DNA, DNA Primers, DNA Replication, DNA-Directed DNA Polymerase, Fluorescent Dyes, Kinetics, Substrate Specificity, Templates, Genetic |
| Abstract | An allylic adenosine triphosphate analog (AATP) was tested as a substrate for commercially available DNA polymerases. All but one of the enzymes assayed incorporated AATP opposite thymidine (T) with concomitant termination of the elongation reaction. A concentration of only 1 microM was sufficient for complete termination of the polymerization reaction for a short template mediated by Ampli Taq DNA polymerase FS (Taq FS). This result suggests that AATP could be used as a 2',3'-dideoxyadenosine-5'-triphosphate (ddA) surrogate. Kinetics of incorporation revealed that AATP was 48 times less efficiently incorporated than ddA. Furthermore, AATP was used in dye-primer sequencing as a substitute for ddA.
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| DOI | 10.1093/nar/27.5.1271 |
| Alternate Journal | Nucleic Acids Res |
| PubMed ID | 9973613 |
| PubMed Central ID | PMC148311 |
| Grant List | HG01745 / HG / NHGRI NIH HHS / United States |