Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis.

TitleAnalysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis.
Publication TypeJournal Article
Year of Publication2000
AuthorsXu, Y, Singh, KV, Qin, X, Murray, BE, Weinstock, GM
JournalInfect Immun
Volume68
Issue2
Pagination815-23
Date Published2000 Feb
ISSN0019-9567
KeywordsAnimals, Enterococcus faecalis, Genes, Bacterial, Humans, Mice, Multigene Family, Mutation, Open Reading Frames, Peritonitis, Polysaccharides, Bacterial, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Transcription, Genetic
Abstract

Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4, encoding a putative glycosyl transferase, and orfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.

Alternate JournalInfect. Immun.
PubMed ID10639451
PubMed Central IDPMC97210
Grant ListAI33516 / AI / NIAID NIH HHS / United States