Analysis of human immunodeficiency virus type 1 integrase mutants.

TitleAnalysis of human immunodeficiency virus type 1 integrase mutants.
Publication TypeJournal Article
Year of Publication1995
AuthorsAnsari-Lari, MA, Donehower, LA, Gibbs, RA
JournalVirology
Volume211
Issue1
Pagination332-5
Date Published1995 Aug 01
ISSN0042-6822
KeywordsBase Sequence, Blotting, Western, Cell Line, Defective Viruses, DNA Nucleotidyltransferases, DNA Primers, Gene Products, gag, Gene Products, pol, Genome, Viral, HIV Reverse Transcriptase, HIV-1, Humans, Integrases, Molecular Sequence Data, Point Mutation, Polymerase Chain Reaction, RNA-Directed DNA Polymerase, Transfection, Viral Proteins
Abstract

The human immunodeficiency virus type-1 (HIV-1) integrase protein (IN) mediates the insertion of linear double-stranded viral DNA into the host genome. Mutations in IN can have different effects on the virus life cycle. In this study, Gag-Pol polyprotein processing, Tat synthesis, and viral replication were investigated in integrase-defective HIV-1 mutants. In the absence of IN synthesis, the Gag-Pol polyprotein stability, packaging, and/or processing was reduced. There was limited expression of Tat observed in IN mutants, but no viral replication.

DOI10.1006/viro.1995.1412
Alternate JournalVirology
PubMed ID7544046
Grant List1P30AI36211 / AI / NIAID NIH HHS / United States
UO1AI30243 / AI / NIAID NIH HHS / United States