Basic principles and technologies for deciphering the genetic map of cancer.

TitleBasic principles and technologies for deciphering the genetic map of cancer.
Publication TypeJournal Article
Year of Publication2009
AuthorsVoidonikolas, G, Kreml, SS, Chen, C, Fisher, WE, F Brunicardi, C, Gibbs, RA, Gingras, M-C
JournalWorld J Surg
Volume33
Issue4
Pagination615-29
Date Published2009 Apr
ISSN0364-2313
KeywordsAdenocarcinoma, DNA Fragmentation, Genes, Neoplasm, Genetic Techniques, Humans, Neoplasms, Nucleic Acid Amplification Techniques, Pancreatic Neoplasms, Polymorphism, Single Nucleotide, Proto-Oncogene Proteins, Proto-Oncogene Proteins p21(ras), ras Proteins, Sequence Analysis, DNA
Abstract

The progress achieved in the field of genomics in recent years is leading medicine to adopt a personalized model in which the knowledge of individual DNA alterations will allow a targeted approach to cancer. Using pancreatic cancer as a model, we discuss herein the fundamentals that need to be considered for the high throughput and global identification of mutations. These include patient-related issues, sample collection, DNA isolation, gene selection, primer design, and sequencing techniques. We also describe the possible applications of the discovery of DNA changes to the approach of this disease and cite preliminary efforts where the knowledge has been translated into the clinical or preclinical setting.

DOI10.1007/s00268-008-9851-y
Alternate JournalWorld J Surg
PubMed ID19115029
PubMed Central IDPMC2924149
Grant ListR13 CA132572 / CA / NCI NIH HHS / United States
R13 CA132572-01 / CA / NCI NIH HHS / United States