To be or not to be - Decoding the Trabecular Meshwork Cell Identity.

TitleTo be or not to be - Decoding the Trabecular Meshwork Cell Identity.
Publication TypeJournal Article
Year of Publication2024
AuthorsTian, A, Baidouri, H, Kim, S, Li, J, Cheng, X, Li, Y, Chen, R, Raghunathan, VK
Date Published2024 Apr 29

The trabecular meshwork within the conventional outflow apparatus is critical in maintaining intraocular pressure homeostasis. studies employing primary cell cultures of the human trabecular meshwork (hTM) have conventionally served as surrogates for investigating the pathobiology of TM dysfunction. Despite its abundant use, translation of outcomes from studies to and/or studies remains a challenge. Given the cell heterogeneity, performing single-cell RNA sequencing comparing primary hTM cell cultures to hTM tissue may provide important insights on cellular identity and translatability, as such an approach has not been reported before. In this study, we assembled a total of 14 primary hTM samples across passages 1-4, including 4 samples from individuals diagnosed with glaucoma. This dataset offers a comprehensive transcriptomic resource of primary hTM scRNA-seq data to study global changes in gene expression in comparison to cells in tissue . We have performed extensive preprocessing and quality control, allowing the research community to access and utilize this public resource.

Alternate JournalbioRxiv
PubMed ID38746421
PubMed Central IDPMC11092480
Grant ListR01 EY018571 / EY / NEI NIH HHS / United States
R01 EY022356 / EY / NEI NIH HHS / United States
R01 EY026048 / EY / NEI NIH HHS / United States
S10 OD032189 / OD / NIH HHS / United States