Biobanking in genomic medicine.

TitleBiobanking in genomic medicine.
Publication TypeJournal Article
Year of Publication2015
AuthorsZhou, JH, Sahin, AA, Myers, JN
JournalArch Pathol Lab Med
Date Published2015 Jun
KeywordsBiological Specimen Banks, Cryopreservation, Gene Expression Profiling, Genomics, Humans, Molecular Medicine, Paraffin Embedding, Quality Control, Specimen Handling, Tissue Fixation

CONTEXT: Genomic medicine requires the identification of biomarkers and therapeutic targets, which in turn, requires high-quality biospecimens. Achieving high-quality biospecimens requires implementing standard operating procedures to control the variations of preanalytic variables in biobanking. Currently, most biobanks do not control the variations of preanalytic variables when collecting, processing, and storing their biospecimens. However, those variations have been shown to affect the quality of biospecimens and gene expression profiling.

OBJECTIVE: To identify evidence-based preanalytic parameters that can be applied and those parameters that need further study.

DATA SOURCES: We searched the Biospecimen Research and PubMed databases using defined key words. We retrieved and reviewed 212 articles obtained through those searches. We included 58 articles (27%) according to our inclusion and exclusion criteria for this review.

CONCLUSION: -Preanalytic variables in biobanking can degrade the quality of biospecimens and alter gene expression profiling. Variables that require further study include the effect of surgical manipulation; the effect of warm ischemia; the allowable duration of delayed specimen processing; the optimal type, duration, and temperature of preservation and fixation; and the optimal storage duration of formalin-fixed, paraffin embedded specimens in a fit-for-purpose approach.

Alternate JournalArch. Pathol. Lab. Med.
PubMed ID26030251
PubMed Central IDPMC4852858
Grant ListT32 CA163185 / CA / NCI NIH HHS / United States
T32CA163185 / CA / NCI NIH HHS / United States

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