Building a comprehensive genomic program for hepatocellular carcinoma.

TitleBuilding a comprehensive genomic program for hepatocellular carcinoma.
Publication TypeJournal Article
Year of Publication2011
AuthorsHarring, TR, Guiteau, JJ, N Nguyen, TT, Cotton, RT, Gingras, M-C, Wheeler, DA, O'Mahony, CA, Gibbs, RA, F Brunicardi, C, Goss, JA
JournalWorld J Surg
Date Published2011 Aug
KeywordsCarcinoma, Hepatocellular, Cell Transformation, Neoplastic, Databases, Nucleic Acid, Disease Progression, Epigenomics, Exome, Forecasting, Gene Expression Regulation, Neoplastic, Genome, Human, Genome-Wide Association Study, Humans, Liver Neoplasms, Precision Medicine, Tissue Banks

BACKGROUND: Hepatocellular carcinoma (HCC) is the most common primary liver cancer, causing approximately 660,000 deaths worldwide annually. The preferred treatment of HCC is surgical resection or orthotopic liver transplantation (OLT) for patients meeting specific criteria. For patients outside these criteria, options are limited and include medical therapy, radiofrequency ablation, chemoembolization, or palliative measures, and these result in poor outcomes. Various centers at Baylor are elucidating the genomics of HCC to improve treatment options, with a focus on three etiologies: hepatitis C virus, hepatitis B virus, and non-viral.METHODS: Through collaborative efforts, we have established an effective specimen biobanking protocol, and we are using several techniques to analyze HCC, including whole genome sequencing, whole exome sequencing, gene-specific analysis, gene expression, and epigenetic analysis.RESULTS: We have completed whole genome sequencing on two patient samples, whole exome sequencing on 47 patient samples, gene-specific analysis on 94 patient samples, gene expression on 4 patient samples, and epigenetic analysis on 1 patient sample.CONCLUSIONS: We hope to use these results to define novel genetic therapeutic strategies that may work in conjunction with surgical approaches to improve long-term patient and graft survival rates in patients with HCC. We also aim to provide a functional framework of a comprehensive program for genomic analysis that may be imitated by other institutions and for other tumors in the global quest toward personalized genomic medicine.

Alternate JournalWorld J Surg
PubMed ID21225260
PubMed Central IDPMC4137964
Grant ListU54 HG003273 / HG / NHGRI NIH HHS / United States
U54-HG003273 / HG / NHGRI NIH HHS / United States
U54-HG004973 / HG / NHGRI NIH HHS / United States

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