Characterization of human genomic yeast artificial chromosome inserts containing hexokinase 1 coding information on chromosome 10.

TitleCharacterization of human genomic yeast artificial chromosome inserts containing hexokinase 1 coding information on chromosome 10.
Publication TypeJournal Article
Year of Publication1992
AuthorsGelb, BD, Worley, KC, Griffin, LD, Adams, V, Chinault, AC, McCabe, ER
JournalBiochem Med Metab Biol
Volume47
Issue3
Pagination265-9
Date Published1992 Jun
ISSN0885-4505
KeywordsAnimals, Base Sequence, Chromosome Mapping, Chromosomes, Fungal, Chromosomes, Human, Pair 10, Cricetinae, DNA, Genome, Human, Hexokinase, Humans, Isoenzymes, Molecular Sequence Data, Polymerase Chain Reaction, Yeasts
Abstract

Hexokinase 1 (HK1) is one of four mammalian HK isoenzymes and maps to human chromosome 10. Two yeast artificial chromosomes (YACs) were identified in the Washington University human YAC library using polymerase chain reaction (PCR) primers designed with knowledge of the human HK1 cDNA sequence. YAC B129B12 is 120 kb in length and maps entirely to chromosome 10. YAC A159D5 is 400 kb in length and appears to have resulted from a recombination of chromosome 10 with non-chromosome 10 material. We report these YACs as potential resources for those interested in HK1 gene organization and mapping, as well as those desiring additional genomic information and markers on chromosome 10.

Alternate JournalBiochem. Med. Metab. Biol.
PubMed ID1627358
Grant List1 F32 GM 13063 / GM / NIGMS NIH HHS / United States
1 P30 HD24064 / HD / NICHD NIH HHS / United States
1 RO1 HD22563 / HD / NICHD NIH HHS / United States