Characterization of the minimal replicator of Kaposi's sarcoma-associated herpesvirus latent origin.

TitleCharacterization of the minimal replicator of Kaposi's sarcoma-associated herpesvirus latent origin.
Publication TypeJournal Article
Year of Publication2005
AuthorsHu, J, Renne, R
JournalJ Virol
Volume79
Issue4
Pagination2637-42
Date Published2005 Feb
ISSN0022-538X
KeywordsAntigens, Viral, DNA Replication, DNA, Viral, Enhancer Elements, Genetic, Herpesvirus 8, Human, Humans, Nuclear Proteins, Replication Origin, Sarcoma, Kaposi, Terminal Repeat Sequences, Virus Latency
Abstract

The latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) binds to two sites within the 801-bp-long terminal repeat (TR) and is the only viral protein required for episomal maintenance. While two or more copies of TR are required for long-term maintenance, a single TR confers LANA-dependent origin activity on plasmid DNA. Deletion mapping revealed a 71-bp-long minimal replicator containing two distinctive sequence elements: LANA binding sites (LBS1/2) and an adjacent 29- to 32-bp-long GC-rich sequence which we termed the replication element. Furthermore, the transcription factor Sp1 can bind to TR outside the minimal replicator and contributes to TR's previously reported enhancer activity.

DOI10.1128/JVI.79.4.2637-2642.2005
Alternate JournalJ. Virol.
PubMed ID15681465
PubMed Central IDPMC546548
Grant ListR01 CA088763 / CA / NCI NIH HHS / United States
R21 CA097939 / CA / NCI NIH HHS / United States
R01 CA 88763 / CA / NCI NIH HHS / United States
R21 CA97939 / CA / NCI NIH HHS / United States