|Title||Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Petrosino, JF, Xiang, Q, Karpathy, SE, Jiang, H, Yerrapragada, S, Liu, Y, Gioia, J, Hemphill, L, Gonzalez, A, Raghavan, TM, Uzman, A, Fox, GE, Highlander, S, Reichard, M, Morton, RJ, Clinkenbeard, KD, Weinstock, GM|
|Date Published||2006 Oct|
|Keywords||Chromosomes, Bacterial, DNA Transposable Elements, DNA, Bacterial, Evolution, Molecular, Francisella tularensis, Gene Rearrangement, Genome, Bacterial, Molecular Sequence Data, Polymorphism, Genetic, Pseudogenes, Recombination, Genetic, Sequence Analysis, DNA, Sequence Homology, Virulence|
The gamma-proteobacterium Francisella tularensis is one of the most infectious human pathogens, and the highly virulent organism F. tularensis subsp. tularensis (type A) and less virulent organism F. tularensis subsp. holarctica (type B) are most commonly associated with significant disease in humans and animals. Here we report the complete genome sequence and annotation for a low-passage type B strain (OSU18) isolated from a dead beaver found near Red Rock, Okla., in 1978. A comparison of the F. tularensis subsp. holarctica sequence with that of F. tularensis subsp. tularensis strain Schu4 (P. Larsson et al., Nat. Genet. 37:153-159, 2005) highlighted genetic differences that may underlie different pathogenicity phenotypes and the evolutionary relationship between type A and type B strains. Despite extensive DNA sequence identity, the most significant difference between type A and type B isolates is the striking amount of genomic rearrangement that exists between the strains. All but two rearrangements can be attributed to homologous recombination occurring between two prominent insertion elements, ISFtu1 and ISFtu2. Numerous pseudogenes have been found in the genomes and are likely contributors to the difference in virulence between the strains. In contrast, no rearrangements have been observed between the OSU18 genome and the genome of the type B live vaccine strain (LVS), and only 448 polymorphisms have been found within non-transposase-coding sequences whose homologs are intact in OSU18. Nonconservative differences between the two strains likely include the LVS attenuating mutation(s).
|Alternate Journal||J Bacteriol|
|PubMed Central ID||PMC1595524|
|Grant List||R21 AI061106 / AI / NIAID NIH HHS / United States |
U54 AI057156 / AI / NIAID NIH HHS / United States
Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence.
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