Clone-array pooled shotgun mapping and sequencing: design and analysis of experiments.

TitleClone-array pooled shotgun mapping and sequencing: design and analysis of experiments.
Publication TypeJournal Article
Year of Publication2003
AuthorsCsuros, M, Li, B, Milosavljevic, A
JournalGenome Inform
Volume14
Pagination186-95
Date Published2003
ISSN0919-9454
KeywordsAnimals, Chromosomes, Artificial, Bacterial, Computational Biology, Computer Simulation, Drosophila melanogaster, Genome, Reproducibility of Results, Research Design, Sequence Alignment
Abstract

This paper studies sequencing and mapping methods that rely solely on pooling and shotgun sequencing of clones. First, we scrutinize and improve the recently proposed Clone-Array Pooled Shotgun Sequencing (CAPSS) method, which delivers a BAC-linked assembly of a whole genome sequence. Secondly, we introduce a novel physical mapping method, called Clone-Array Pooled Shotgun Mapping (CAPS-MAP), which computes the physical ordering of BACs in a random library. Both CAPSS and CAPS-MAP construct subclone libraries from pooled genomic BAC clones.
Alternate JournalGenome Inform
PubMed ID15706533
Grant ListR01 HG02583-01 / HG / NHGRI NIH HHS / United States
U01 RR18464 / RR / NCRR NIH HHS / United States

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