A clone-array pooled shotgun strategy for sequencing large genomes.

TitleA clone-array pooled shotgun strategy for sequencing large genomes.
Publication TypeJournal Article
Year of Publication2001
AuthorsCai, WW, Chen, R, Gibbs, RA, Bradley, A
JournalGenome Res
Volume11
Issue10
Pagination1619-23
Date Published2001 Oct
ISSN1088-9051
KeywordsAnimals, Computer Simulation, Genome, Humans, Mice, Oligonucleotide Array Sequence Analysis, Software
Abstract

A simplified strategy for sequencing large genomes is proposed. Clone-Array Pooled Shotgun Sequencing (CAPSS) is based on pooling rows and columns of arrayed genomic clones, for shotgun library construction. Random sequences are accumulated, and the data are processed by sequential comparison of rows and columns to assemble the sequence of clones at points of intersection. Compared with either a clone-by-clone approach or whole-genome shotgun sequencing, CAPSS requires relatively few library constructions and only minimal computational power for a complete genome assembly. The strategy is suitable for sequencing large genomes for which there are no sequence-ready maps, but for which relatively high resolution STS maps and highly redundant BAC libraries are available. It is immediately applicable to the sequencing of mouse, rat, zebrafish, and other important genomes, and can be managed in a cooperative fashion to take advantage of a distributed international DNA sequencing capacity.

DOI10.1101/gr.198101
Alternate JournalGenome Res.
PubMed ID11591638