Cloning and identification of a microRNA cluster within the latency-associated region of Kaposi's sarcoma-associated herpesvirus.

TitleCloning and identification of a microRNA cluster within the latency-associated region of Kaposi's sarcoma-associated herpesvirus.
Publication TypeJournal Article
Year of Publication2005
AuthorsSamols, MA, Hu, J, Skalsky, RL, Renne, R
JournalJ Virol
Volume79
Issue14
Pagination9301-5
Date Published2005 Jul
ISSN0022-538X
KeywordsCloning, Molecular, Herpesvirus 8, Human, Humans, MicroRNAs, Virus Latency
Abstract

MicroRNAs (miRNAs) are small, noncoding regulatory RNA molecules that bind to 3' untranslated regions (UTRs) of mRNAs to either prevent their translation or induce their degradation. Previously identified in a variety of organisms ranging from plants to mammals, miRNAs are also now known to be produced by viruses. The human gammaherpesvirus Epstein-Barr virus has been shown to encode miRNAs, which potentially regulate both viral and cellular genes. To determine whether Kaposi's sarcoma-associated herpesvirus (KSHV) encodes miRNAs, we cloned small RNAs from KSHV-positive primary effusion lymphoma-derived cells and endothelial cells. Sequence analysis revealed 11 isolated RNAs of 19 to 23 bases in length that perfectly align with KSHV. Surprisingly, all candidate miRNAs mapped to a single genomic locale within the latency-associated region of KSHV. These data suggest that viral and host cellular gene expression may be regulated by miRNAs during both latent and lytic KSHV replication.

DOI10.1128/JVI.79.14.9301-9305.2005
Alternate JournalJ. Virol.
PubMed ID15994824
PubMed Central IDPMC1168752
Grant ListR21 CA097939 / CA / NCI NIH HHS / United States
R21 CA97939 / CA / NCI NIH HHS / United States
R01 CA 88763 / CA / NCI NIH HHS / United States
R01 CA088763 / CA / NCI NIH HHS / United States
T32 GM007250 / GM / NIGMS NIH HHS / United States