|Title||Comparative analysis of the bovine MHC class IIb sequence identifies inversion breakpoints and three unexpected genes.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Childers, CP, Newkirk, HL, Honeycutt, DA, Ramlachan, N, Muzny, DM, Sodergren, E, Gibbs, RA, Weinstock, GM, Womack, JE, Skow, LC|
|Date Published||2006 Apr|
|Keywords||Animals, Cattle, Chromosome Inversion, Chromosomes, Mammalian, Contig Mapping, Evolution, Molecular, Genes, MHC Class II, Histones, Humans, Molecular Sequence Data, Sequence Analysis, DNA|
The bovine major histocompatibility complex (MHC) or BoLA is organized differently from typical mammalian MHCs in that a large portion of the class II region, called class IIb, has been transposed to a position near the centromere on bovine chromosome 23. Gene mapping indicated that the rearrangement resulted from a single inversion, but the boundaries and gene content of the inverted segment have not been fully determined. Here, we report the genomic sequence of BoLA IIb. Comparative sequence analysis with the human MHC revealed that the proximal inversion breakpoint occurred approximately 2.5 kb from the 3' end of the glutamate-cysteine ligase, catalytic subunit (GCLC) locus and that the distal breakpoint occurred about 2 kb from the 5' end from a divergent class IIDRbeta-like sequence designated DSB. Gene content, order and orientation of BoLA IIb are consistent with the single inversion hypothesis when compared with the corresponding region of the human class II MHC (HLA class II). Differences with HLA include the presence of a single histone H2B gene located between the proteasome subunit, beta type, 9 (PSMB9) and DMB loci and a duplicated TAP2 with a variant splice site. BoLA IIb spans approximately 450 kb DNA, with 20 apparently intact genes and no obvious pseudogenes. The region contains 227 simple sequence repeats (SSRs) and approximately 167 kb of retroviral-related repetitive DNA. Nineteen of the 20 genes identified in silico are supported by bovine EST data indicating that the functional gene content of BoLA IIb has not been diminished because it has been transposed from the remainder of BoLA genes.
|Alternate Journal||Anim. Genet.|