|Title||Comparison of cell adhesion molecule expression between glioblastoma multiforme and autologous normal brain tissue.|
|Publication Type||Journal Article|
|Year of Publication||1995|
|Authors||Gingras, M-C, Roussel, E, Bruner, JM, Branch, CD, Moser, RP|
|Date Published||1995 Mar|
|Keywords||Antigens, CD, Antigens, Tumor-Associated, Carbohydrate, Brain Chemistry, Brain Neoplasms, Carrier Proteins, CD58 Antigens, Cell Adhesion Molecules, Endothelium, Vascular, Glioblastoma, Humans, Hyaluronan Receptors, Immunohistochemistry, Integrins, Intercellular Adhesion Molecule-1, Membrane Glycoproteins, Receptors, Cell Surface, Receptors, Lymphocyte Homing|
We investigated glioblastoma multiforme (GBM) for a pattern of consistent alterations in cell adhesion molecules (CAM) expression that might distinguish tumor from normal autologous brain tissue. We used frozen section immunohistochemistry with anti-CAM and computerized image analysis to quantify staining intensity which we expressed as relative intensity units (RIU). Our results showed that normal brain tissue generally did not express alpha 1 beta 1, intercellular CAM-1 (ICAM-1), and sialylated Lewisx, slightly expressed alpha 2, alpha 4, alpha 5, alpha 6 beta 1, alpha v beta 3, lymphocyte function-associated antigen-3 (LFA-3), Lewisx, sialylated LewisLewisx, had a good expression of alpha 3 beta 1 and CD44, and strongly expressed neural CAM (NCAM). GBM expressed alpha 2, alpha 3, alpha 5, alpha 6 beta 1, alpha v beta 3, ICAM-1, LFA-3, CD44, Lewisx, sialylated Lewisx, and sialylated LewisLewisx significantly higher (2-11-fold RIU) than normal brain tissue. ICAM-1 and LFA-3 were the most distinctive markers of GBM. The small blood vessel endothelial cells of the normal brain and the GBM showed a few differences. The tumor endothelium expression of alpha 2 beta 1, alpha 4 beta 1, and LFA-3 RIU appeared twice higher than in normal endothelium and alpha 6 beta 1 showed an average of 40% RIU decrease in comparison to normal. These results show that the expression of several CAM is consistently altered in GBM and its microvasculature when compared with autologous normal brain tissue.
|Alternate Journal||J. Neuroimmunol.|