Comparison of cell adhesion molecule expression between glioblastoma multiforme and autologous normal brain tissue.

TitleComparison of cell adhesion molecule expression between glioblastoma multiforme and autologous normal brain tissue.
Publication TypeJournal Article
Year of Publication1995
AuthorsGingras, M-C, Roussel, E, Bruner, JM, Branch, CD, Moser, RP
JournalJ Neuroimmunol
Volume57
Issue1-2
Pagination143-53
Date Published1995 Mar
ISSN0165-5728
KeywordsAntigens, CD, Antigens, Tumor-Associated, Carbohydrate, Brain Chemistry, Brain Neoplasms, Carrier Proteins, CD58 Antigens, Cell Adhesion Molecules, Endothelium, Vascular, Glioblastoma, Humans, Hyaluronan Receptors, Immunohistochemistry, Integrins, Intercellular Adhesion Molecule-1, Membrane Glycoproteins, Receptors, Cell Surface, Receptors, Lymphocyte Homing
Abstract

We investigated glioblastoma multiforme (GBM) for a pattern of consistent alterations in cell adhesion molecules (CAM) expression that might distinguish tumor from normal autologous brain tissue. We used frozen section immunohistochemistry with anti-CAM and computerized image analysis to quantify staining intensity which we expressed as relative intensity units (RIU). Our results showed that normal brain tissue generally did not express alpha 1 beta 1, intercellular CAM-1 (ICAM-1), and sialylated Lewisx, slightly expressed alpha 2, alpha 4, alpha 5, alpha 6 beta 1, alpha v beta 3, lymphocyte function-associated antigen-3 (LFA-3), Lewisx, sialylated LewisLewisx, had a good expression of alpha 3 beta 1 and CD44, and strongly expressed neural CAM (NCAM). GBM expressed alpha 2, alpha 3, alpha 5, alpha 6 beta 1, alpha v beta 3, ICAM-1, LFA-3, CD44, Lewisx, sialylated Lewisx, and sialylated LewisLewisx significantly higher (2-11-fold RIU) than normal brain tissue. ICAM-1 and LFA-3 were the most distinctive markers of GBM. The small blood vessel endothelial cells of the normal brain and the GBM showed a few differences. The tumor endothelium expression of alpha 2 beta 1, alpha 4 beta 1, and LFA-3 RIU appeared twice higher than in normal endothelium and alpha 6 beta 1 showed an average of 40% RIU decrease in comparison to normal. These results show that the expression of several CAM is consistently altered in GBM and its microvasculature when compared with autologous normal brain tissue.

Alternate JournalJ. Neuroimmunol.
PubMed ID7535788