|Title||Complete sequence of a 38.4-kb human cosmid insert containing the polymorphic marker DXS455 from Xq28.|
|Publication Type||Journal Article|
|Year of Publication||1995|
|Authors||Andersson, B, Lu, F, Muzny, DM, Warren, ST, Gibbs, RA|
|Keywords||Bacteriophage M13, Base Sequence, Cosmids, DNA, Gene Library, Genetic Markers, Humans, Minisatellite Repeats, Molecular Sequence Data, Repetitive Sequences, Nucleic Acid, X Chromosome|
The complete DNA sequence of a cosmid mapping to human Xq28 (DXS455) has been determined using a shotgun approach (genbank accession number: L31948). The cosmid insert is 38.4 kb in length, and contains several repetitive sequences, including a highly repetitive region located in a fragment previously shown to contain a polymorphic VNTR locus, as well as short di- and tri-nucleotide repeats. In addition to the previously known VNTR locus, a CA-repeat and an unusual 800-bp repetitive region have been found to be polymorphic. The repeated sequence mapping to the site of the VNTR locus spans 480 bp in the cosmid. In addition to several AT-dinucleotide stretches, this repeat has four copies of a 25 bp repeat unit and flanking sequences similar to this 25-mer. No expressed sequences have so far been identified in this cosmid.
|Alternate Journal||DNA Seq.|
|Grant List||R01 HG00823-01A1 / HG / NHGRI NIH HHS / United States|