The completion of the Mammalian Gene Collection (MGC).

TitleThe completion of the Mammalian Gene Collection (MGC).
Publication TypeJournal Article
Year of Publication2009
AuthorsTemple, G, Gerhard, DS, Rasooly, R, Feingold, EA, Good, PJ, Robinson, C, Mandich, A, Derge, JG, Lewis, J, Shoaf, D, Collins, FS, Jang, W, Wagner, L, Shenmen, CM, Misquitta, L, Schaefer, CF, Buetow, KH, Bonner, TI, Yankie, L, Ward, M, Phan, L, Astashyn, A, Brown, G, Farrell, C, Hart, J, Landrum, M, Maidak, BL, Murphy, M, Murphy, T, Rajput, B, Riddick, L, Webb, D, Weber, J, Wu, W, Pruitt, KD, Maglott, D, Siepel, A, Brejova, B, Diekhans, M, Harte, R, Baertsch, R, Kent, J, Haussler, D, Brent, M, Langton, L, Comstock, CLG, Stevens, M, Wei, C, van Baren, MJ, Salehi-Ashtiani, K, Murray, RR, Ghamsari, L, Mello, E, Lin, C, Pennacchio, C, Schreiber, K, Shapiro, N, Marsh, A, Pardes, E, Moore, T, Lebeau, A, Muratet, M, Simmons, B, Kloske, D, Sieja, S, Hudson, J, Sethupathy, P, Brownstein, M, Bhat, N, Lazar, J, Jacob, H, Gruber, CE, Smith, MR, McPherson, J, Garcia, AM, Gunaratne, PH, Wu, J, Muzny, D, Gibbs, RA, Young, AC, Bouffard, GG, Blakesley, RW, Mullikin, J, Green, ED, Dickson, MC, Rodriguez, AC, Grimwood, J, Schmutz, J, Myers, RM, Hirst, M, Zeng, T, Tse, K, Moksa, M, Deng, M, Ma, K, Mah, D, Pang, J, Taylor, G, Chuah, E, Deng, A, Fichter, K, Go, A, Lee, S, Wang, J, Griffith, M, Morin, R, Moore, RA, Mayo, M, Munro, S, Wagner, S, Jones, SJM, Holt, RA, Marra, MA, Lu, S, Yang, S, Hartigan, J, Graf, M, Wagner, R, Letovksy, S, Pulido, JC, Robison, K, Esposito, D, Hartley, J, Wall, VE, Hopkins, RF, Ohara, O, Wiemann, S
Corporate AuthorsMGC Project Team
JournalGenome Res
Date Published2009 Dec
KeywordsAnimals, Cloning, Molecular, Computational Biology, DNA, DNA, Complementary, Gene Library, Genes, Humans, Mammals, Mice, National Institutes of Health (U.S.), Rats, Reverse Transcriptase Polymerase Chain Reaction, United States

Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.

Alternate JournalGenome Res
PubMed ID19767417
PubMed Central IDPMC2792178
Grant ListU54 HG003273 / HG / NHGRI NIH HHS / United States
N01-C0-12400 / / PHS HHS / United States

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