Concatenation cDNA sequencing for transcriptome analysis.

TitleConcatenation cDNA sequencing for transcriptome analysis.
Publication TypeJournal Article
Year of Publication2003
AuthorsGunaratne, PH, Wu, JQian, Garcia, AM, Hulyk, S, Worley, KC, Margolin, JF, Gibbs, RA
JournalC R Biol
Volume326
Issue10-11
Pagination971-7
Date Published2003 Oct-Nov
ISSN1631-0691
KeywordsCloning, Molecular, DNA, Complementary, DNA, Concatenated, Genetic Techniques, Humans, Sequence Analysis, DNA, Transcription, Genetic
Abstract

We describe a high-throughput cDNA sequencing pipeline (http://www.hgsc.bcm.tmc.edu/projects/cdna) built in response to the emerging need for rapid sequencing of large cDNA collections. Using this strategy cDNA inserts are purified and joined through concatenation into large molecules. These 'pseudo-BACs' are subjected to random shotgun sequencing whereby the majority of cDNA inserts in the pool are sequenced. Using this concatenation cDNA sequencing platform, we have contributed more than 13000 full-length cDNA sequences from human and mouse to the Mammalian Gene Collection (MGC).

Alternate JournalC. R. Biol.
PubMed ID14744103
Grant List20X182A / / PHS HHS / United States
5 UO1 CA 80200 / CA / NCI NIH HHS / United States