De Novo Genome Assembly for the Coppery Titi Monkey (Plecturocebus cupreus): An Emerging Nonhuman Primate Model for Behavioral Research.

TitleDe Novo Genome Assembly for the Coppery Titi Monkey (Plecturocebus cupreus): An Emerging Nonhuman Primate Model for Behavioral Research.
Publication TypeJournal Article
Year of Publication2024
AuthorsPfeifer, SP, Baxter, A, Savidge, LE, Sedlazeck, FJ, Bales, KL
JournalGenome Biol Evol
Volume16
Issue5
Date Published2024 May 02
ISSN1759-6653
KeywordsAnimals, Genome, Genomics, Models, Animal, Pitheciidae
Abstract

The coppery titi monkey (Plecturocebus cupreus) is an emerging nonhuman primate model system for behavioral and neurobiological research. At the same time, the almost entire absence of genomic resources for the species has hampered insights into the genetic underpinnings of the phenotypic traits of interest. To facilitate future genotype-to-phenotype studies, we here present a high-quality, fully annotated de novo genome assembly for the species with chromosome-length scaffolds spanning the autosomes and chromosome X (scaffold N50 = 130.8 Mb), constructed using data obtained from several orthologous short- and long-read sequencing and scaffolding techniques. With a base-level accuracy of ∼99.99% in chromosome-length scaffolds as well as benchmarking universal single-copy ortholog and k-mer completeness scores of >99.0% and 95.1% at the genome level, this assembly represents one of the most complete Pitheciidae genomes to date, making it an invaluable resource for comparative evolutionary genomics research to improve our understanding of lineage-specific changes underlying adaptive traits as well as deleterious mutations associated with disease.

DOI10.1093/gbe/evae108
Alternate JournalGenome Biol Evol
PubMed ID38758096
PubMed Central IDPMC11140417
Grant ListNIH P51OD011107 / / California National Primate Research Center /
P51 OD011107 / OD / NIH HHS / United States
R01 MH125411 / MH / NIMH NIH HHS / United States
S10 OD010786 / OD / NIH HHS / United States
DEB-2045343 / / National Science Foundation CAREER /
/ / Good Nature Institute /
R35GM151008 / NH / NIH HHS / United States
R35 GM151008 / GM / NIGMS NIH HHS / United States
R01 HD092055 / HD / NICHD NIH HHS / United States

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