Designing new microsatellite markers for linkage and population genetic analyses in rhesus macaques and other nonhuman primates.

TitleDesigning new microsatellite markers for linkage and population genetic analyses in rhesus macaques and other nonhuman primates.
Publication TypeJournal Article
Year of Publication2006
AuthorsRaveendran, M, R Harris, A, Milosavljevic, A, Johnson, Z, Shelledy, W, Cameron, J, Rogers, J
JournalGenomics
Volume88
Issue6
Pagination706-710
Date Published2006 Dec
ISSN0888-7543
KeywordsAnimals, Chromosome Mapping, Genetic Markers, Genetic Techniques, Genetics, Population, Humans, Macaca mulatta, Microsatellite Repeats, Papio hamadryas, Pedigree, Polymorphism, Genetic
Abstract

Identification of polymorphic microsatellite loci in nonhuman primates is useful for various biomedical and evolutionary studies of these species. Prior methods for identifying microsatellites in nonhuman primates are inefficient. We describe a new strategy for marker development that uses the available whole genome sequence for rhesus macaques. Fifty-four novel rhesus-derived microsatellites were genotyped in large pedigrees of rhesus monkeys. Linkage analysis was used to place 51 of these loci into the existing rhesus linkage map. In addition, we find that microsatellites identified this way are polymorphic in other Old World monkeys such as baboons. This approach to marker development is more efficient than previous methods and produces polymorphisms with known locations in the rhesus genome assembly. Finally, we propose a nomenclature system that can be used for rhesus-derived microsatellites genotyped in any species or for novel loci derived from the genome sequence of any nonhuman primate.

DOI10.1016/j.ygeno.2006.08.009
Alternate JournalGenomics
PubMed ID17010566
Grant ListR24-RR015383 / RR / NCRR NIH HHS / United States
R24 RR015383 / RR / NCRR NIH HHS / United States
R01 MH062568 / MH / NIMH NIH HHS / United States
R01-RR08781 / RR / NCRR NIH HHS / United States
MH62568 / MH / NIMH NIH HHS / United States
R01 RR008781 / RR / NCRR NIH HHS / United States