Detection of single DNA base differences by competitive oligonucleotide priming.

TitleDetection of single DNA base differences by competitive oligonucleotide priming.
Publication TypeJournal Article
Year of Publication1989
AuthorsGibbs, RA, Nguyen, PN, Caskey, CT
JournalNucleic Acids Res
Volume17
Issue7
Pagination2437-48
Date Published1989 Apr 11
ISSN0305-1048
KeywordsAnimals, Base Sequence, Binding, Competitive, DNA, DNA, Single-Stranded, DNA-Directed DNA Polymerase, Gene Amplification, Mice, Mice, Mutant Strains, Molecular Sequence Data, Mutation, Oligonucleotides, Taq Polymerase, Templates, Genetic
Abstract

Synthetic DNA oligonucleotides can serve as efficient primers for DNA synthesis even when there is a single base mismatch between the primers and the corresponding DNA template. However, when the primer-template annealing is carried out with a mixture of primers and at low stringency the binding of a perfectly matched primer is strongly favored relative to a primer differing by a single base. This primer competition is observed over a range of oligonucleotide sizes from twelve to sixteen bases and with a variety of base mismatches. When coupled with the polymerase chain reaction, for the amplification of specific DNA sequences, competitive oligonucleotide priming provides a simple general strategy for the detection of single DNA base differences.

Alternate JournalNucleic Acids Res.
PubMed ID2717399
PubMed Central IDPMC317634
Grant ListDK31428 / DK / NIDDK NIH HHS / United States