Differential regulation and xenobiotic induction of tandem P450 monooxygenase genes pc-1 (CYP63A1) and pc-2 (CYP63A2) in the white-rot fungus Phanerochaete chrysosporium.

TitleDifferential regulation and xenobiotic induction of tandem P450 monooxygenase genes pc-1 (CYP63A1) and pc-2 (CYP63A2) in the white-rot fungus Phanerochaete chrysosporium.
Publication TypeJournal Article
Year of Publication2004
AuthorsDoddapaneni, H, Yadav, JS
JournalAppl Microbiol Biotechnol
Volume65
Issue5
Pagination559-65
Date Published2004 Oct
ISSN0175-7598
KeywordsAdaptation, Physiological, Benz(a)Anthracenes, Benzo(a)pyrene, Carbohydrate Metabolism, Culture Media, Cytochrome P-450 Enzyme System, Enzyme Activators, Fungal Proteins, Gene Expression Regulation, Enzymologic, Naphthalenes, Oxygen, Phanerochaete, Phenanthrenes, Polycyclic Aromatic Hydrocarbons, Pyrenes, RNA, Fungal, RNA, Messenger, Temperature, Transcription, Genetic, Xenobiotics
Abstract

The two tandem P450 monooxygenase genes (pc-1 and pc-2) reported by us earlier in Phanerochaete chrysosporium were investigated for their regulation under nutrient-limited and nutrient-rich culture conditions. Transcript analysis based on real-time quantitative RT-PCR showed higher expression of pc-1 in defined low-nitrogen and pc-2 in defined high-nitrogen media, with maximum expression on day 4, indicating that the two genes, though tandemly linked, are regulated in a non-coordinate manner. Transcript levels of pc-1 and pc-2 were differentially influenced by the type of carbon source, incubation temperature, and oxygenation. Both genes were inducible by organic xenobiotic chemicals. Of the 42 xenobiotics tested in nutrient-rich cultures, pc-1 transcription was induced 2.12(+/-0.40)-fold to 6.27(+/-0.48)-fold in the presence of 19 compounds and pc-2 transcription was induced 2.00(+/-0.73)-fold to 29.39(+/-9.40)-fold in the presence of 22 compounds. Particularly, it is significant that both pc-1 and pc-2 are induced by polycyclic aromatic hydrocarbons (PAHs) of varying ring size, including naphthalene (4.35+/-0.09, 6.02+/-1.39), phenanthrene (2.82+/-0.12, 2.14+/-0.61), pyrene (3.93+/-0.01, 1.0+/-0.12), benzanthracene (1.67+/-0.03, 6.08+/-1.50), and benzo(a)pyrene (1.55+/-0.01, 5.54+/-2.75) respectively. This study constitutes the first report on the identification of P450 genes in a fungus that are responsive to environmentally significant pollutant chemicals (PAHs, DDT, long-chain alkyl phenols) and lignin derivatives.

DOI10.1007/s00253-004-1645-z
Alternate JournalAppl Microbiol Biotechnol
PubMed ID15378295
Grant ListR01-ES10210 / ES / NIEHS NIH HHS / United States