Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.

TitleDivergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.
Publication TypeJournal Article
Year of Publication2017
AuthorsTcw, J, Carvalho, CMB, Yuan, B, Gu, S, Altheimer, AN, McCarthy, S, Malhotra, D, Sebat, J, Siegel, AJ, Rudolph, U, Lupski, JR, Levy, DL, Brennand, KJ
JournalStem Cell Reports
Volume8
Issue3
Pagination519-528
Date Published2017 Mar 14
ISSN2213-6711
Abstract

In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.

DOI10.1016/j.stemcr.2017.01.010
Alternate JournalStem Cell Reports
PubMed ID28216146
PubMed Central IDPMC5355568
Grant ListR21 MH104505 / MH / NIMH NIH HHS / United States
R01 MH101454 / MH / NIMH NIH HHS / United States
R01 NS058529 / NS / NINDS NIH HHS / United States
R21 MH097470 / MH / NIMH NIH HHS / United States
R21 MH105732 / MH / NIMH NIH HHS / United States