Title | Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Tcw, J, Carvalho, CMB, Yuan, B, Gu, S, Altheimer, AN, McCarthy, S, Malhotra, D, Sebat, J, Siegel, AJ, Rudolph, U, Lupski, JR, Levy, DL, Brennand, KJ |
Journal | Stem Cell Reports |
Volume | 8 |
Issue | 3 |
Pagination | 519-528 |
Date Published | 2017 Mar 14 |
ISSN | 2213-6711 |
Keywords | Chromosome Duplication, Chromosomes, Human, Chromosomes, Human, Pair 9, Comparative Genomic Hybridization, Genetic Association Studies, Genetic Markers, Genetic Predisposition to Disease, Heterozygote, Humans, Induced Pluripotent Stem Cells, Matrix Attachment Regions, Mosaicism, Psychotic Disorders, Trisomy |
Abstract | In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures. |
DOI | 10.1016/j.stemcr.2017.01.010 |
Alternate Journal | Stem Cell Reports |
PubMed ID | 28216146 |
PubMed Central ID | PMC5355568 |
Grant List | R21 MH104505 / MH / NIMH NIH HHS / United States R01 MH101454 / MH / NIMH NIH HHS / United States R01 NS058529 / NS / NINDS NIH HHS / United States R21 MH097470 / MH / NIMH NIH HHS / United States R21 MH105732 / MH / NIMH NIH HHS / United States |
Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.
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