Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.

TitleDivergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.
Publication TypeJournal Article
Year of Publication2017
AuthorsTcw, J, Carvalho, CMB, Yuan, B, Gu, S, Altheimer, AN, McCarthy, S, Malhotra, D, Sebat, J, Siegel, AJ, Rudolph, U, Lupski, JR, Levy, DL, Brennand, KJ
JournalStem Cell Reports
Date Published2017 Feb 09

In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.

Alternate JournalStem Cell Reports
PubMed ID28216146
Grant ListR01 MH101454 / MH / NIMH NIH HHS / United States