%0 Journal Article %J J Neuroimmunol %D 1995 %T Comparison of cell adhesion molecule expression between glioblastoma multiforme and autologous normal brain tissue. %A Marie-Claude Gingras %A Roussel, E %A Bruner, J M %A Branch, C D %A Moser, R P %K Antigens, CD %K Antigens, Tumor-Associated, Carbohydrate %K Brain Chemistry %K Brain Neoplasms %K Carrier Proteins %K CD58 Antigens %K Cell Adhesion Molecules %K Endothelium, Vascular %K Glioblastoma %K Humans %K Hyaluronan Receptors %K Immunohistochemistry %K Integrins %K Intercellular Adhesion Molecule-1 %K Membrane Glycoproteins %K Receptors, Cell Surface %K Receptors, Lymphocyte Homing %X

We investigated glioblastoma multiforme (GBM) for a pattern of consistent alterations in cell adhesion molecules (CAM) expression that might distinguish tumor from normal autologous brain tissue. We used frozen section immunohistochemistry with anti-CAM and computerized image analysis to quantify staining intensity which we expressed as relative intensity units (RIU). Our results showed that normal brain tissue generally did not express alpha 1 beta 1, intercellular CAM-1 (ICAM-1), and sialylated Lewisx, slightly expressed alpha 2, alpha 4, alpha 5, alpha 6 beta 1, alpha v beta 3, lymphocyte function-associated antigen-3 (LFA-3), Lewisx, sialylated LewisLewisx, had a good expression of alpha 3 beta 1 and CD44, and strongly expressed neural CAM (NCAM). GBM expressed alpha 2, alpha 3, alpha 5, alpha 6 beta 1, alpha v beta 3, ICAM-1, LFA-3, CD44, Lewisx, sialylated Lewisx, and sialylated LewisLewisx significantly higher (2-11-fold RIU) than normal brain tissue. ICAM-1 and LFA-3 were the most distinctive markers of GBM. The small blood vessel endothelial cells of the normal brain and the GBM showed a few differences. The tumor endothelium expression of alpha 2 beta 1, alpha 4 beta 1, and LFA-3 RIU appeared twice higher than in normal endothelium and alpha 6 beta 1 showed an average of 40% RIU decrease in comparison to normal. These results show that the expression of several CAM is consistently altered in GBM and its microvasculature when compared with autologous normal brain tissue.

%B J Neuroimmunol %V 57 %P 143-53 %8 1995 Mar %G eng %N 1-2 %1 https://www.ncbi.nlm.nih.gov/pubmed/7535788?dopt=Abstract %R 10.1016/0165-5728(94)00178-q