%0 Journal Article %J J Virol %D 1996 %T Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection. %A Ansari-Lari, M A %A Gibbs, R A %K Base Sequence %K Cell Line %K Fusion Proteins, gag-pol %K HIV Long Terminal Repeat %K HIV Reverse Transcriptase %K HIV-1 %K Molecular Sequence Data %K Mutation %K Retroelements %K RNA-Directed DNA Polymerase %K Virion %K Virus Assembly %X

The normal reverse transcription of retroviral RNA is a complex process which depends on the orchestration of several steps throughout the virus life cycle. During the assembly of retroviruses, reverse transcriptase (RT) is directed into the virion as a component of the Gag-Pol polyprotein. In the maturation of the Gag-Pol polyprotein of human immunodeficiency virus type 1 (HIV-1), cleavage by the viral protease occurs during viral budding. After infection, reverse transcription of viral RNA into double-stranded DNA is completed in the cytoplasm of the infected cell. In this study, the processing and reverse transcription of HIV-1 have been examined by separate expression of mature HIV-1 RT and proviral molecules bearing RT mutations. The effects of RT expression in trans during virion release and after viral entry were investigated. Constitutive expression of HIV-1 RT was established in CD4- and non-CD4-expressing cells via the coexpression of its individual subunits, and three HIV-1 RT mutant constructs were generated. The results indicate that a bona fide RT trans complementation does not occur during virion release or after infection. However, after infection of an RT-expressing cell with a high titer RT-defective virus, intracellular reverse transcription can be detected.

%B J Virol %V 70 %P 3870-5 %8 1996 Jun %G eng %N 6 %1 https://www.ncbi.nlm.nih.gov/pubmed/8648723?dopt=Abstract %R 10.1128/JVI.70.6.3870-3875.1996