%0 Journal Article %J Nature %D 2006 %T The DNA sequence, annotation and analysis of human chromosome 3. %A Donna M Muzny %A Steven E Scherer %A Kaul, Rajinder %A Wang, Jing %A Yu, Jun %A Sudbrak, Ralf %A Buhay, Christian J %A Rui Chen %A Cree, Andrew %A Ding, Yan %A Dugan-Rocha, Shannon %A Gill, Rachel %A Gunaratne, Preethi %A R. Alan Harris %A Hawes, Alicia C %A Hernandez, Judith %A Hodgson, Anne V %A Hume, Jennifer %A Jackson, Andrew %A Ziad Khan %A Kovar-Smith, Christie %A Lewis, Lora R %A Lozado, Ryan J %A Metzker, Michael L %A Milosavljevic, Aleksandar %A Miner, George R %A Morgan, Margaret B %A Nazareth, Lynne V %A Scott, Graham %A Sodergren, Erica %A Song, Xing-Zhi %A Steffen, David %A Wei, Sharon %A David A Wheeler %A Wright, Mathew W %A Kim C Worley %A Yuan, Ye %A Zhang, Zhengdong %A Adams, Charles Q %A Ansari-Lari, M Ali %A Ayele, Mulu %A Brown, Mary J %A Chen, Guan %A Chen, Zhijian %A Clendenning, James %A Clerc-Blankenburg, Kerstin P %A Chen, Runsheng %A Chen, Zhu %A Davis, Clay %A Delgado, Oliver %A Dinh, Huyen H %A Dong, Wei %A Draper, Heather %A Ernst, Stephen %A Fu, Gang %A Gonzalez-Garay, Manuel L %A Garcia, Dawn K %A Gillett, Will %A Gu, Jun %A Hao, Bailin %A Haugen, Eric %A Havlak, Paul %A He, Xin %A Hennig, Steffen %A Hu, Songnian %A Huang, Wei %A Jackson, Laronda R %A Jacob, Leni S %A Kelly, Susan H %A Kube, Michael %A Levy, Ruth %A Li, Zhangwan %A Liu, Bin %A Liu, Jing %A Liu, Wen %A Lu, Jing %A Maheshwari, Manjula %A Nguyen, Bao-Viet %A Okwuonu, Geoffrey O %A Palmeiri, Anthony %A Pasternak, Shiran %A Perez, Lesette M %A Phelps, Karen A %A Plopper, Farah J H %A Qiang, Boqin %A Raymond, Christopher %A Rodriguez, Ruben %A Saenphimmachak, Channakhone %A Santibanez, Jireh %A Shen, Hua %A Shen, Yan %A Subramanian, Sandhya %A Tabor, Paul E %A Verduzco, Daniel %A Waldron, Lenée %A Wang, Jian %A Wang, Jun %A Wang, Qiaoyan %A Williams, Gabrielle A %A Wong, Gane K-S %A Yao, Zhijian %A Zhang, Jingkun %A Zhang, Xiuqing %A Zhao, Guoping %A Zhou, Jianling %A Zhou, Yang %A Nelson, David %A Lehrach, Hans %A Reinhardt, Richard %A Naylor, Susan L %A Yang, Huanming %A Olson, Maynard %A Weinstock, George %A Richard A Gibbs %K Animals %K Base Sequence %K Chromosome Breakage %K Chromosome Inversion %K Chromosomes, Human, Pair 3 %K Contig Mapping %K CpG Islands %K DNA, Complementary %K Evolution, Molecular %K Expressed Sequence Tags %K Human Genome Project %K Humans %K Macaca mulatta %K Molecular Sequence Data %K Pan troglodytes %K Sequence Analysis, DNA %K Synteny %X

After the completion of a draft human genome sequence, the International Human Genome Sequencing Consortium has proceeded to finish and annotate each of the 24 chromosomes comprising the human genome. Here we describe the sequencing and analysis of human chromosome 3, one of the largest human chromosomes. Chromosome 3 comprises just four contigs, one of which currently represents the longest unbroken stretch of finished DNA sequence known so far. The chromosome is remarkable in having the lowest rate of segmental duplication in the genome. It also includes a chemokine receptor gene cluster as well as numerous loci involved in multiple human cancers such as the gene encoding FHIT, which contains the most common constitutive fragile site in the genome, FRA3B. Using genomic sequence from chimpanzee and rhesus macaque, we were able to characterize the breakpoints defining a large pericentric inversion that occurred some time after the split of Homininae from Ponginae, and propose an evolutionary history of the inversion.

%B Nature %V 440 %P 1194-8 %8 2006 Apr 27 %G eng %N 7088 %1 https://www.ncbi.nlm.nih.gov/pubmed/16641997?dopt=Abstract %R 10.1038/nature04728 %0 Journal Article %J Nature %D 2005 %T The DNA sequence of the human X chromosome. %A Ross, Mark T %A Grafham, Darren V %A Coffey, Alison J %A Scherer, Steven %A McLay, Kirsten %A Muzny, Donna %A Platzer, Matthias %A Howell, Gareth R %A Burrows, Christine %A Bird, Christine P %A Frankish, Adam %A Lovell, Frances L %A Howe, Kevin L %A Ashurst, Jennifer L %A Fulton, Robert S %A Sudbrak, Ralf %A Wen, Gaiping %A Jones, Matthew C %A Hurles, Matthew E %A Andrews, T Daniel %A Scott, Carol E %A Searle, Stephen %A Ramser, Juliane %A Whittaker, Adam %A Deadman, Rebecca %A Carter, Nigel P %A Hunt, Sarah E %A Chen, Rui %A Cree, Andrew %A Gunaratne, Preethi %A Havlak, Paul %A Hodgson, Anne %A Metzker, Michael L %A Richards, Stephen %A Scott, Graham %A Steffen, David %A Sodergren, Erica %A Wheeler, David A %A Worley, Kim C %A Ainscough, Rachael %A Ambrose, Kerrie D %A Ansari-Lari, M Ali %A Aradhya, Swaroop %A Ashwell, Robert I S %A Babbage, Anne K %A Bagguley, Claire L %A Ballabio, Andrea %A Banerjee, Ruby %A Barker, Gary E %A Barlow, Karen F %A Barrett, Ian P %A Bates, Karen N %A Beare, David M %A Beasley, Helen %A Beasley, Oliver %A Beck, Alfred %A Bethel, Graeme %A Blechschmidt, Karin %A Brady, Nicola %A Bray-Allen, Sarah %A Bridgeman, Anne M %A Brown, Andrew J %A Brown, Mary J %A Bonnin, David %A Bruford, Elspeth A %A Buhay, Christian %A Burch, Paula %A Burford, Deborah %A Burgess, Joanne %A Burrill, Wayne %A Burton, John %A Bye, Jackie M %A Carder, Carol %A Carrel, Laura %A Chako, Joseph %A Chapman, Joanne C %A Chavez, Dean %A Chen, Ellson %A Chen, Guan %A Chen, Yuan %A Chen, Zhijian %A Chinault, Craig %A Ciccodicola, Alfredo %A Clark, Sue Y %A Clarke, Graham %A Clee, Chris M %A Clegg, Sheila %A Clerc-Blankenburg, Kerstin %A Clifford, Karen %A Cobley, Vicky %A Cole, Charlotte G %A Conquer, Jen S %A Corby, Nicole %A Connor, Richard E %A David, Robert %A Davies, Joy %A Davis, Clay %A Davis, John %A Delgado, Oliver %A Deshazo, Denise %A Dhami, Pawandeep %A Ding, Yan %A Dinh, Huyen %A Dodsworth, Steve %A Draper, Heather %A Dugan-Rocha, Shannon %A Dunham, Andrew %A Dunn, Matthew %A Durbin, K James %A Dutta, Ireena %A Eades, Tamsin %A Ellwood, Matthew %A Emery-Cohen, Alexandra %A Errington, Helen %A Evans, Kathryn L %A Faulkner, Louisa %A Francis, Fiona %A Frankland, John %A Fraser, Audrey E %A Galgoczy, Petra %A Gilbert, James %A Gill, Rachel %A Glöckner, Gernot %A Gregory, Simon G %A Gribble, Susan %A Griffiths, Coline %A Grocock, Russell %A Gu, Yanghong %A Gwilliam, Rhian %A Hamilton, Cerissa %A Hart, Elizabeth A %A Hawes, Alicia %A Heath, Paul D %A Heitmann, Katja %A Hennig, Steffen %A Hernandez, Judith %A Hinzmann, Bernd %A Ho, Sarah %A Hoffs, Michael %A Howden, Phillip J %A Huckle, Elizabeth J %A Hume, Jennifer %A Hunt, Paul J %A Hunt, Adrienne R %A Isherwood, Judith %A Jacob, Leni %A Johnson, David %A Jones, Sally %A De Jong, Pieter J %A Joseph, Shirin S %A Keenan, Stephen %A Kelly, Susan %A Kershaw, Joanne K %A Khan, Ziad %A Kioschis, Petra %A Klages, Sven %A Knights, Andrew J %A Kosiura, Anna %A Kovar-Smith, Christie %A Laird, Gavin K %A Langford, Cordelia %A Lawlor, Stephanie %A Leversha, Margaret %A Lewis, Lora %A Liu, Wen %A Lloyd, Christine %A Lloyd, David M %A Loulseged, Hermela %A Loveland, Jane E %A Lovell, Jamieson D %A Lozado, Ryan %A Lu, Jing %A Lyne, Rachael %A Ma, Jie %A Maheshwari, Manjula %A Matthews, Lucy H %A McDowall, Jennifer %A McLaren, Stuart %A McMurray, Amanda %A Meidl, Patrick %A Meitinger, Thomas %A Milne, Sarah %A Miner, George %A Mistry, Shailesh L %A Morgan, Margaret %A Morris, Sidney %A Müller, Ines %A Mullikin, James C %A Nguyen, Ngoc %A Nordsiek, Gabriele %A Nyakatura, Gerald %A O'Dell, Christopher N %A Okwuonu, Geoffery %A Palmer, Sophie %A Pandian, Richard %A Parker, David %A Parrish, Julia %A Pasternak, Shiran %A Patel, Dina %A Pearce, Alex V %A Pearson, Danita M %A Pelan, Sarah E %A Perez, Lesette %A Porter, Keith M %A Ramsey, Yvonne %A Reichwald, Kathrin %A Rhodes, Susan %A Ridler, Kerry A %A Schlessinger, David %A Schueler, Mary G %A Sehra, Harminder K %A Shaw-Smith, Charles %A Shen, Hua %A Sheridan, Elizabeth M %A Shownkeen, Ratna %A Skuce, Carl D %A Smith, Michelle L %A Sotheran, Elizabeth C %A Steingruber, Helen E %A Steward, Charles A %A Storey, Roy %A Swann, R Mark %A Swarbreck, David %A Tabor, Paul E %A Taudien, Stefan %A Taylor, Tineace %A Teague, Brian %A Thomas, Karen %A Thorpe, Andrea %A Timms, Kirsten %A Tracey, Alan %A Trevanion, Steve %A Tromans, Anthony C %A d'Urso, Michele %A Verduzco, Daniel %A Villasana, Donna %A Waldron, Lenée %A Wall, Melanie %A Wang, Qiaoyan %A Warren, James %A Warry, Georgina L %A Wei, Xuehong %A West, Anthony %A Whitehead, Siobhan L %A Whiteley, Mathew N %A Wilkinson, Jane E %A Willey, David L %A Williams, Gabrielle %A Williams, Leanne %A Williamson, Angela %A Williamson, Helen %A Wilming, Laurens %A Woodmansey, Rebecca L %A Wray, Paul W %A Yen, Jennifer %A Zhang, Jingkun %A Zhou, Jianling %A Zoghbi, Huda %A Zorilla, Sara %A Buck, David %A Reinhardt, Richard %A Poustka, Annemarie %A Rosenthal, André %A Lehrach, Hans %A Meindl, Alfons %A Minx, Patrick J %A Hillier, LaDeana W %A Willard, Huntington F %A Wilson, Richard K %A Waterston, Robert H %A Rice, Catherine M %A Vaudin, Mark %A Coulson, Alan %A Nelson, David L %A Weinstock, George %A Sulston, John E %A Durbin, Richard %A Hubbard, Tim %A Gibbs, Richard A %A Beck, Stephan %A Rogers, Jane %A Bentley, David R %K Animals %K Antigens, Neoplasm %K Centromere %K Chromosomes, Human, X %K Chromosomes, Human, Y %K Contig Mapping %K Crossing Over, Genetic %K Dosage Compensation, Genetic %K Evolution, Molecular %K Female %K Genetic Linkage %K Genetics, Medical %K Genomics %K Humans %K Male %K Polymorphism, Single Nucleotide %K Repetitive Sequences, Nucleic Acid %K RNA %K Sequence Analysis, DNA %K Sequence Homology, Nucleic Acid %K Testis %X

The human X chromosome has a unique biology that was shaped by its evolution as the sex chromosome shared by males and females. We have determined 99.3% of the euchromatic sequence of the X chromosome. Our analysis illustrates the autosomal origin of the mammalian sex chromosomes, the stepwise process that led to the progressive loss of recombination between X and Y, and the extent of subsequent degradation of the Y chromosome. LINE1 repeat elements cover one-third of the X chromosome, with a distribution that is consistent with their proposed role as way stations in the process of X-chromosome inactivation. We found 1,098 genes in the sequence, of which 99 encode proteins expressed in testis and in various tumour types. A disproportionately high number of mendelian diseases are documented for the X chromosome. Of this number, 168 have been explained by mutations in 113 X-linked genes, which in many cases were characterized with the aid of the DNA sequence.

%B Nature %V 434 %P 325-37 %8 2005 Mar 17 %G eng %N 7031 %1 https://www.ncbi.nlm.nih.gov/pubmed/15772651?dopt=Abstract %R 10.1038/nature03440 %0 Journal Article %J Nature %D 2004 %T Genome sequence of the Brown Norway rat yields insights into mammalian evolution. %A Gibbs, Richard A %A Weinstock, George M %A Metzker, Michael L %A Muzny, Donna M %A Sodergren, Erica J %A Scherer, Steven %A Scott, Graham %A Steffen, David %A Worley, Kim C %A Burch, Paula E %A Okwuonu, Geoffrey %A Hines, Sandra %A Lewis, Lora %A DeRamo, Christine %A Delgado, Oliver %A Dugan-Rocha, Shannon %A Miner, George %A Morgan, Margaret %A Hawes, Alicia %A Gill, Rachel %A Holt, Robert A %A Adams, Mark D %A Amanatides, Peter G %A Baden-Tillson, Holly %A Barnstead, Mary %A Chin, Soo %A Evans, Cheryl A %A Ferriera, Steve %A Fosler, Carl %A Glodek, Anna %A Gu, Zhiping %A Jennings, Don %A Kraft, Cheryl L %A Nguyen, Trixie %A Pfannkoch, Cynthia M %A Sitter, Cynthia %A Sutton, Granger G %A Venter, J Craig %A Woodage, Trevor %A Smith, Douglas %A Lee, Hong-Mei %A Gustafson, Erik %A Cahill, Patrick %A Kana, Arnold %A Doucette-Stamm, Lynn %A Weinstock, Keith %A Fechtel, Kim %A Weiss, Robert B %A Dunn, Diane M %A Green, Eric D %A Blakesley, Robert W %A Bouffard, Gerard G %A De Jong, Pieter J %A Osoegawa, Kazutoyo %A Zhu, Baoli %A Marra, Marco %A Schein, Jacqueline %A Bosdet, Ian %A Fjell, Chris %A Jones, Steven %A Krzywinski, Martin %A Mathewson, Carrie %A Siddiqui, Asim %A Wye, Natasja %A McPherson, John %A Zhao, Shaying %A Fraser, Claire M %A Shetty, Jyoti %A Shatsman, Sofiya %A Geer, Keita %A Chen, Yixin %A Abramzon, Sofyia %A Nierman, William C %A Havlak, Paul H %A Chen, Rui %A Durbin, K James %A Simons, Rain %A Ren, Yanru %A Song, Xing-Zhi %A Li, Bingshan %A Liu, Yue %A Qin, Xiang %A Cawley, Simon %A Worley, Kim C %A Cooney, A J %A D'Souza, Lisa M %A Martin, Kirt %A Wu, Jia Qian %A Gonzalez-Garay, Manuel L %A Jackson, Andrew R %A Kalafus, Kenneth J %A McLeod, Michael P %A Milosavljevic, Aleksandar %A Virk, Davinder %A Volkov, Andrei %A Wheeler, David A %A Zhang, Zhengdong %A Bailey, Jeffrey A %A Eichler, Evan E %A Tuzun, Eray %A Birney, Ewan %A Mongin, Emmanuel %A Ureta-Vidal, Abel %A Woodwark, Cara %A Zdobnov, Evgeny %A Bork, Peer %A Suyama, Mikita %A Torrents, David %A Alexandersson, Marina %A Trask, Barbara J %A Young, Janet M %A Huang, Hui %A Wang, Huajun %A Xing, Heming %A Daniels, Sue %A Gietzen, Darryl %A Schmidt, Jeanette %A Stevens, Kristian %A Vitt, Ursula %A Wingrove, Jim %A Camara, Francisco %A Mar Albà, M %A Abril, Josep F %A Guigó, Roderic %A Smit, Arian %A Dubchak, Inna %A Rubin, Edward M %A Couronne, Olivier %A Poliakov, Alexander %A Hübner, Norbert %A Ganten, Detlev %A Goesele, Claudia %A Hummel, Oliver %A Kreitler, Thomas %A Lee, Young-Ae %A Monti, Jan %A Schulz, Herbert %A Zimdahl, Heike %A Himmelbauer, Heinz %A Lehrach, Hans %A Jacob, Howard J %A Bromberg, Susan %A Gullings-Handley, Jo %A Jensen-Seaman, Michael I %A Kwitek, Anne E %A Lazar, Jozef %A Pasko, Dean %A Tonellato, Peter J %A Twigger, Simon %A Ponting, Chris P %A Duarte, Jose M %A Rice, Stephen %A Goodstadt, Leo %A Beatson, Scott A %A Emes, Richard D %A Winter, Eitan E %A Webber, Caleb %A Brandt, Petra %A Nyakatura, Gerald %A Adetobi, Margaret %A Chiaromonte, Francesca %A Elnitski, Laura %A Eswara, Pallavi %A Hardison, Ross C %A Hou, Minmei %A Kolbe, Diana %A Makova, Kateryna %A Miller, Webb %A Nekrutenko, Anton %A Riemer, Cathy %A Schwartz, Scott %A Taylor, James %A Yang, Shan %A Zhang, Yi %A Lindpaintner, Klaus %A Andrews, T Dan %A Caccamo, Mario %A Clamp, Michele %A Clarke, Laura %A Curwen, Valerie %A Durbin, Richard %A Eyras, Eduardo %A Searle, Stephen M %A Cooper, Gregory M %A Batzoglou, Serafim %A Brudno, Michael %A Sidow, Arend %A Stone, Eric A %A Venter, J Craig %A Payseur, Bret A %A Bourque, Guillaume %A López-Otín, Carlos %A Puente, Xose S %A Chakrabarti, Kushal %A Chatterji, Sourav %A Dewey, Colin %A Pachter, Lior %A Bray, Nicolas %A Yap, Von Bing %A Caspi, Anat %A Tesler, Glenn %A Pevzner, Pavel A %A Haussler, David %A Roskin, Krishna M %A Baertsch, Robert %A Clawson, Hiram %A Furey, Terrence S %A Hinrichs, Angie S %A Karolchik, Donna %A Kent, William J %A Rosenbloom, Kate R %A Trumbower, Heather %A Weirauch, Matt %A Cooper, David N %A Stenson, Peter D %A Ma, Bin %A Brent, Michael %A Arumugam, Manimozhiyan %A Shteynberg, David %A Copley, Richard R %A Taylor, Martin S %A Riethman, Harold %A Mudunuri, Uma %A Peterson, Jane %A Guyer, Mark %A Felsenfeld, Adam %A Old, Susan %A Mockrin, Stephen %A Collins, Francis %K Animals %K Base Composition %K Centromere %K Chromosomes, Mammalian %K CpG Islands %K DNA Transposable Elements %K DNA, Mitochondrial %K Evolution, Molecular %K Gene Duplication %K Genome %K Genomics %K Humans %K Introns %K Male %K Mice %K Models, Molecular %K Mutagenesis %K Polymorphism, Single Nucleotide %K Rats %K Rats, Inbred BN %K Regulatory Sequences, Nucleic Acid %K Retroelements %K RNA Splice Sites %K RNA, Untranslated %K Sequence Analysis, DNA %K Telomere %X

The laboratory rat (Rattus norvegicus) is an indispensable tool in experimental medicine and drug development, having made inestimable contributions to human health. We report here the genome sequence of the Brown Norway (BN) rat strain. The sequence represents a high-quality 'draft' covering over 90% of the genome. The BN rat sequence is the third complete mammalian genome to be deciphered, and three-way comparisons with the human and mouse genomes resolve details of mammalian evolution. This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution.

%B Nature %V 428 %P 493-521 %8 2004 Apr 01 %G eng %N 6982 %1 https://www.ncbi.nlm.nih.gov/pubmed/15057822?dopt=Abstract %R 10.1038/nature02426 %0 Journal Article %J Genome Res %D 2004 %T Integrated and sequence-ordered BAC- and YAC-based physical maps for the rat genome. %A Krzywinski, Martin %A Wallis, John %A Gösele, Claudia %A Bosdet, Ian %A Chiu, Readman %A Graves, Tina %A Hummel, Oliver %A Layman, Dan %A Mathewson, Carrie %A Wye, Natasja %A Zhu, Baoli %A Albracht, Derek %A Asano, Jennifer %A Barber, Sarah %A Brown-John, Mabel %A Chan, Susanna %A Chand, Steve %A Cloutier, Alison %A Davito, Jonathon %A Fjell, Chris %A Gaige, Tony %A Ganten, Detlev %A Girn, Noreen %A Guggenheimer, Kurtis %A Himmelbauer, Heinz %A Kreitler, Thomas %A Leach, Stephen %A Lee, Darlene %A Lehrach, Hans %A Mayo, Michael %A Mead, Kelly %A Olson, Teika %A Pandoh, Pawan %A Prabhu, Anna-Liisa %A Shin, Heesun %A Tänzer, Simone %A Thompson, Jason %A Tsai, Miranda %A Walker, Jason %A Yang, George %A Sekhon, Mandeep %A Hillier, LaDeana %A Zimdahl, Heike %A Marziali, Andre %A Osoegawa, Kazutoyo %A Zhao, Shaying %A Siddiqui, Asim %A De Jong, Pieter J %A Warren, Wes %A Mardis, Elaine %A McPherson, John D %A Wilson, Richard %A Hübner, Norbert %A Jones, Steven %A Marra, Marco %A Schein, Jacqueline %K Animals %K Automation %K Chromosomes %K Chromosomes, Artificial, Bacterial %K Chromosomes, Artificial, Yeast %K Cloning, Molecular %K Computational Biology %K Contig Mapping %K DNA Fingerprinting %K Genetic Markers %K Genome %K Physical Chromosome Mapping %K Polymerase Chain Reaction %K Rats %K Sequence Analysis, DNA %X

As part of the effort to sequence the genome of Rattus norvegicus, we constructed a physical map comprised of fingerprinted bacterial artificial chromosome (BAC) clones from the CHORI-230 BAC library. These BAC clones provide approximately 13-fold redundant coverage of the genome and have been assembled into 376 fingerprint contigs. A yeast artificial chromosome (YAC) map was also constructed and aligned with the BAC map via fingerprinted BAC and P1 artificial chromosome clones (PACs) sharing interspersed repetitive sequence markers with the YAC-based physical map. We have annotated 95% of the fingerprint map clones in contigs with coordinates on the version 3.1 rat genome sequence assembly, using BAC-end sequences and in silico mapping methods. These coordinates have allowed anchoring 358 of the 376 fingerprint map contigs onto the sequence assembly. Of these, 324 contigs are anchored to rat genome sequences localized to chromosomes, and 34 contigs are anchored to unlocalized portions of the rat sequence assembly. The remaining 18 contigs, containing 54 clones, still require placement. The fingerprint map is a high-resolution integrative data resource that provides genome-ordered associations among BAC, YAC, and PAC clones and the assembled sequence of the rat genome.

%B Genome Res %V 14 %P 766-79 %8 2004 Apr %G eng %N 4 %1 https://www.ncbi.nlm.nih.gov/pubmed/15060021?dopt=Abstract %R 10.1101/gr.2336604