%0 Journal Article %J Genome Res %D 2009 %T The completion of the Mammalian Gene Collection (MGC). %A Temple, Gary %A Gerhard, Daniela S %A Rasooly, Rebekah %A Feingold, Elise A %A Good, Peter J %A Robinson, Cristen %A Mandich, Allison %A Derge, Jeffrey G %A Lewis, Jeanne %A Shoaf, Debonny %A Collins, Francis S %A Jang, Wonhee %A Wagner, Lukas %A Shenmen, Carolyn M %A Misquitta, Leonie %A Schaefer, Carl F %A Buetow, Kenneth H %A Bonner, Tom I %A Yankie, Linda %A Ward, Ming %A Phan, Lon %A Astashyn, Alex %A Brown, Garth %A Farrell, Catherine %A Hart, Jennifer %A Landrum, Melissa %A Maidak, Bonnie L %A Murphy, Michael %A Murphy, Terence %A Rajput, Bhanu %A Riddick, Lillian %A Webb, David %A Weber, Janet %A Wu, Wendy %A Pruitt, Kim D %A Maglott, Donna %A Siepel, Adam %A Brejova, Brona %A Diekhans, Mark %A Harte, Rachel %A Baertsch, Robert %A Kent, Jim %A Haussler, David %A Brent, Michael %A Langton, Laura %A Comstock, Charles L G %A Stevens, Michael %A Wei, Chaochun %A van Baren, Marijke J %A Salehi-Ashtiani, Kourosh %A Murray, Ryan R %A Ghamsari, Lila %A Mello, Elizabeth %A Lin, Chenwei %A Pennacchio, Christa %A Schreiber, Kirsten %A Shapiro, Nicole %A Marsh, Amber %A Pardes, Elizabeth %A Moore, Troy %A Lebeau, Anita %A Muratet, Mike %A Simmons, Blake %A Kloske, David %A Sieja, Stephanie %A Hudson, James %A Sethupathy, Praveen %A Brownstein, Michael %A Bhat, Narayan %A Lazar, Joseph %A Jacob, Howard %A Gruber, Chris E %A Smith, Mark R %A McPherson, John %A Garcia, Angela M %A Gunaratne, Preethi H %A Wu, Jiaqian %A Muzny, Donna %A Gibbs, Richard A %A Young, Alice C %A Bouffard, Gerard G %A Blakesley, Robert W %A Mullikin, Jim %A Green, Eric D %A Dickson, Mark C %A Rodriguez, Alex C %A Grimwood, Jane %A Schmutz, Jeremy %A Myers, Richard M %A Hirst, Martin %A Zeng, Thomas %A Tse, Kane %A Moksa, Michelle %A Deng, Merinda %A Ma, Kevin %A Mah, Diana %A Pang, Johnson %A Taylor, Greg %A Chuah, Eric %A Deng, Athena %A Fichter, Keith %A Go, Anne %A Lee, Stephanie %A Wang, Jing %A Griffith, Malachi %A Morin, Ryan %A Moore, Richard A %A Mayo, Michael %A Munro, Sarah %A Wagner, Susan %A Jones, Steven J M %A Holt, Robert A %A Marra, Marco A %A Lu, Sun %A Yang, Shuwei %A Hartigan, James %A Graf, Marcus %A Wagner, Ralf %A Letovksy, Stanley %A Pulido, Jacqueline C %A Robison, Keith %A Esposito, Dominic %A Hartley, James %A Wall, Vanessa E %A Hopkins, Ralph F %A Ohara, Osamu %A Wiemann, Stefan %K Animals %K Cloning, Molecular %K Computational Biology %K DNA %K DNA, Complementary %K Gene Library %K Genes %K Humans %K Mammals %K Mice %K National Institutes of Health (U.S.) %K Rats %K Reverse Transcriptase Polymerase Chain Reaction %K United States %X

Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.

%B Genome Res %V 19 %P 2324-33 %8 2009 Dec %G eng %N 12 %1 https://www.ncbi.nlm.nih.gov/pubmed/19767417?dopt=Abstract %R 10.1101/gr.095976.109 %0 Journal Article %J Genome Res %D 2004 %T The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). %A Gerhard, Daniela S %A Wagner, Lukas %A Feingold, Elise A %A Shenmen, Carolyn M %A Grouse, Lynette H %A Schuler, Greg %A Klein, Steven L %A Old, Susan %A Rasooly, Rebekah %A Good, Peter %A Guyer, Mark %A Peck, Allison M %A Derge, Jeffery G %A Lipman, David %A Collins, Francis S %A Jang, Wonhee %A Sherry, Steven %A Feolo, Mike %A Misquitta, Leonie %A Lee, Eduardo %A Rotmistrovsky, Kirill %A Greenhut, Susan F %A Schaefer, Carl F %A Buetow, Kenneth %A Bonner, Tom I %A Haussler, David %A Kent, Jim %A Kiekhaus, Mark %A Furey, Terry %A Brent, Michael %A Prange, Christa %A Schreiber, Kirsten %A Shapiro, Nicole %A Bhat, Narayan K %A Hopkins, Ralph F %A Hsie, Florence %A Driscoll, Tom %A Soares, M Bento %A Casavant, Tom L %A Scheetz, Todd E %A Brown-stein, Michael J %A Usdin, Ted B %A Toshiyuki, Shiraki %A Carninci, Piero %A Piao, Yulan %A Dudekula, Dawood B %A Ko, Minoru S H %A Kawakami, Koichi %A Suzuki, Yutaka %A Sugano, Sumio %A Gruber, C E %A Smith, M R %A Simmons, Blake %A Moore, Troy %A Waterman, Richard %A Johnson, Stephen L %A Ruan, Yijun %A Wei, Chia Lin %A Mathavan, S %A Gunaratne, Preethi H %A Wu, Jiaqian %A Garcia, Angela M %A Hulyk, Stephen W %A Fuh, Edwin %A Yuan, Ye %A Sneed, Anna %A Kowis, Carla %A Hodgson, Anne %A Muzny, Donna M %A McPherson, John %A Gibbs, Richard A %A Fahey, Jessica %A Helton, Erin %A Ketteman, Mark %A Madan, Anuradha %A Rodrigues, Stephanie %A Sanchez, Amy %A Whiting, Michelle %A Madari, Anup %A Young, Alice C %A Wetherby, Keith D %A Granite, Steven J %A Kwong, Peggy N %A Brinkley, Charles P %A Pearson, Russell L %A Bouffard, Gerard G %A Blakesly, Robert W %A Green, Eric D %A Dickson, Mark C %A Rodriguez, Alex C %A Grimwood, Jane %A Schmutz, Jeremy %A Myers, Richard M %A Butterfield, Yaron S N %A Griffith, Malachi %A Griffith, Obi L %A Krzywinski, Martin I %A Liao, Nancy %A Morin, Ryan %A Palmquist, Diana %A Petrescu, Anca S %A Skalska, Ursula %A Smailus, Duane E %A Stott, Jeff M %A Schnerch, Angelique %A Schein, Jacqueline E %A Jones, Steven J M %A Holt, Robert A %A Baross, Agnes %A Marra, Marco A %A Clifton, Sandra %A Makowski, Kathryn A %A Bosak, Stephanie %A Malek, Joel %K Animals %K Cloning, Molecular %K Computational Biology %K DNA Primers %K DNA, Complementary %K Gene Library %K Humans %K Mice %K National Institutes of Health (U.S.) %K Open Reading Frames %K Rats %K United States %K Xenopus laevis %K Zebrafish %X

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline.

%B Genome Res %V 14 %P 2121-7 %8 2004 Oct %G eng %N 10B %1 https://www.ncbi.nlm.nih.gov/pubmed/15489334?dopt=Abstract %R 10.1101/gr.2596504