%0 Journal Article %J PLoS One %D 2014 %T Prospective associations of coronary heart disease loci in African Americans using the MetaboChip: the PAGE study. %A Franceschini, Nora %A Hu, Yijuan %A Reiner, Alex P %A Buyske, Steven %A Nalls, Mike %A Yanek, Lisa R %A Li, Yun %A Hindorff, Lucia A %A Cole, Shelley A %A Howard, Barbara V %A Stafford, Jeanette M %A Carty, Cara L %A Sethupathy, Praveen %A Martin, Lisa W %A Lin, Dan-Yu %A Johnson, Karen C %A Becker, Lewis C %A North, Kari E %A Dehghan, Abbas %A Bis, Joshua C %A Liu, Yongmei %A Greenland, Philip %A Manson, JoAnn E %A Maeda, Nobuyo %A Garcia, Melissa %A Harris, Tamara B %A Becker, Diane M %A O'Donnell, Christopher %A Heiss, Gerardo %A Kooperberg, Charles %A Eric Boerwinkle %K Adaptor Proteins, Vesicular Transport %K Black or African American %K Coronary Disease %K Female %K Genetic Association Studies %K Genetic Predisposition to Disease %K Humans %K Male %K Oligonucleotide Array Sequence Analysis %K Polymorphism, Single Nucleotide %K Prospective Studies %K Proto-Oncogene Proteins c-myc %X

BACKGROUND: Coronary heart disease (CHD) is a leading cause of morbidity and mortality in African Americans. However, there is a paucity of studies assessing genetic determinants of CHD in African Americans. We examined the association of published variants in CHD loci with incident CHD, attempted to fine map these loci, and characterize novel variants influencing CHD risk in African Americans.

METHODS AND RESULTS: Up to 8,201 African Americans (including 546 first CHD events) were genotyped using the MetaboChip array in the Atherosclerosis Risk in Communities (ARIC) study and Women's Health Initiative (WHI). We tested associations using Cox proportional hazard models in sex- and study-stratified analyses and combined results using meta-analysis. Among 44 validated CHD loci available in the array, we replicated and fine-mapped the SORT1 locus, and showed same direction of effects as reported in studies of individuals of European ancestry for SNPs in 22 additional published loci. We also identified a SNP achieving array wide significance (MYC: rs2070583, allele frequency 0.02, P = 8.1 × 10(-8)), but the association did not replicate in an additional 8,059 African Americans (577 events) from the WHI, HealthABC and GeneSTAR studies, and in a meta-analysis of 5 cohort studies of European ancestry (24,024 individuals including 1,570 cases of MI and 2,406 cases of CHD) from the CHARGE Consortium.

CONCLUSIONS: Our findings suggest that some CHD loci previously identified in individuals of European ancestry may be relevant to incident CHD in African Americans.

%B PLoS One %V 9 %P e113203 %8 2014 %G eng %N 12 %1 https://www.ncbi.nlm.nih.gov/pubmed/25542012?dopt=Abstract %R 10.1371/journal.pone.0113203 %0 Journal Article %J Genome Res %D 2009 %T The completion of the Mammalian Gene Collection (MGC). %A Temple, Gary %A Gerhard, Daniela S %A Rasooly, Rebekah %A Feingold, Elise A %A Good, Peter J %A Robinson, Cristen %A Mandich, Allison %A Derge, Jeffrey G %A Lewis, Jeanne %A Shoaf, Debonny %A Collins, Francis S %A Jang, Wonhee %A Wagner, Lukas %A Shenmen, Carolyn M %A Misquitta, Leonie %A Schaefer, Carl F %A Buetow, Kenneth H %A Bonner, Tom I %A Yankie, Linda %A Ward, Ming %A Phan, Lon %A Astashyn, Alex %A Brown, Garth %A Farrell, Catherine %A Hart, Jennifer %A Landrum, Melissa %A Maidak, Bonnie L %A Murphy, Michael %A Murphy, Terence %A Rajput, Bhanu %A Riddick, Lillian %A Webb, David %A Weber, Janet %A Wu, Wendy %A Pruitt, Kim D %A Maglott, Donna %A Siepel, Adam %A Brejova, Brona %A Diekhans, Mark %A Harte, Rachel %A Baertsch, Robert %A Kent, Jim %A Haussler, David %A Brent, Michael %A Langton, Laura %A Comstock, Charles L G %A Stevens, Michael %A Wei, Chaochun %A van Baren, Marijke J %A Salehi-Ashtiani, Kourosh %A Murray, Ryan R %A Ghamsari, Lila %A Mello, Elizabeth %A Lin, Chenwei %A Pennacchio, Christa %A Schreiber, Kirsten %A Shapiro, Nicole %A Marsh, Amber %A Pardes, Elizabeth %A Moore, Troy %A Lebeau, Anita %A Muratet, Mike %A Simmons, Blake %A Kloske, David %A Sieja, Stephanie %A Hudson, James %A Sethupathy, Praveen %A Brownstein, Michael %A Bhat, Narayan %A Lazar, Joseph %A Jacob, Howard %A Gruber, Chris E %A Smith, Mark R %A McPherson, John %A Garcia, Angela M %A Gunaratne, Preethi H %A Wu, Jiaqian %A Muzny, Donna %A Gibbs, Richard A %A Young, Alice C %A Bouffard, Gerard G %A Blakesley, Robert W %A Mullikin, Jim %A Green, Eric D %A Dickson, Mark C %A Rodriguez, Alex C %A Grimwood, Jane %A Schmutz, Jeremy %A Myers, Richard M %A Hirst, Martin %A Zeng, Thomas %A Tse, Kane %A Moksa, Michelle %A Deng, Merinda %A Ma, Kevin %A Mah, Diana %A Pang, Johnson %A Taylor, Greg %A Chuah, Eric %A Deng, Athena %A Fichter, Keith %A Go, Anne %A Lee, Stephanie %A Wang, Jing %A Griffith, Malachi %A Morin, Ryan %A Moore, Richard A %A Mayo, Michael %A Munro, Sarah %A Wagner, Susan %A Jones, Steven J M %A Holt, Robert A %A Marra, Marco A %A Lu, Sun %A Yang, Shuwei %A Hartigan, James %A Graf, Marcus %A Wagner, Ralf %A Letovksy, Stanley %A Pulido, Jacqueline C %A Robison, Keith %A Esposito, Dominic %A Hartley, James %A Wall, Vanessa E %A Hopkins, Ralph F %A Ohara, Osamu %A Wiemann, Stefan %K Animals %K Cloning, Molecular %K Computational Biology %K DNA %K DNA, Complementary %K Gene Library %K Genes %K Humans %K Mammals %K Mice %K National Institutes of Health (U.S.) %K Rats %K Reverse Transcriptase Polymerase Chain Reaction %K United States %X

Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.

%B Genome Res %V 19 %P 2324-33 %8 2009 Dec %G eng %N 12 %1 https://www.ncbi.nlm.nih.gov/pubmed/19767417?dopt=Abstract %R 10.1101/gr.095976.109