%0 Journal Article %J Insect Biochem Mol Biol %D 2016 %T Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta. %A Kanost, Michael R %A Arrese, Estela L %A Cao, Xiaolong %A Chen, Yun-Ru %A Chellapilla, Sanjay %A Goldsmith, Marian R %A Grosse-Wilde, Ewald %A Heckel, David G %A Herndon, Nicolae %A Jiang, Haobo %A Papanicolaou, Alexie %A Qu, Jiaxin %A Soulages, Jose L %A Vogel, Heiko %A Walters, James %A Waterhouse, Robert M %A Ahn, Seung-Joon %A Almeida, Francisca C %A An, Chunju %A Aqrawi, Peshtewani %A Bretschneider, Anne %A Bryant, William B %A Bucks, Sascha %A Chao, Hsu %A Chevignon, Germain %A Christen, Jayne M %A Clarke, David F %A Dittmer, Neal T %A Ferguson, Laura C F %A Garavelou, Spyridoula %A Gordon, Karl H J %A Gunaratna, Ramesh T %A Han, Yi %A Hauser, Frank %A He, Yan %A Heidel-Fischer, Hanna %A Hirsh, Ariana %A Hu, Yingxia %A Jiang, Hongbo %A Kalra, Divya %A Klinner, Christian %A König, Christopher %A Kovar, Christie %A Kroll, Ashley R %A Kuwar, Suyog S %A Lee, Sandy L %A Lehman, Rüdiger %A Li, Kai %A Li, Zhaofei %A Liang, Hanquan %A Lovelace, Shanna %A Lu, Zhiqiang %A Mansfield, Jennifer H %A McCulloch, Kyle J %A Mathew, Tittu %A Morton, Brian %A Muzny, Donna M %A Neunemann, David %A Ongeri, Fiona %A Pauchet, Yannick %A Pu, Ling-Ling %A Pyrousis, Ioannis %A Rao, Xiang-Jun %A Redding, Amanda %A Roesel, Charles %A Sánchez-Gracia, Alejandro %A Schaack, Sarah %A Shukla, Aditi %A Tetreau, Guillaume %A Wang, Yang %A Xiong, Guang-Hua %A Traut, Walther %A Walsh, Tom K %A Worley, Kim C %A Wu, Di %A Wu, Wenbi %A Wu, Yuan-Qing %A Zhang, Xiufeng %A Zou, Zhen %A Zucker, Hannah %A Briscoe, Adriana D %A Burmester, Thorsten %A Clem, Rollie J %A Feyereisen, René %A Grimmelikhuijzen, Cornelis J P %A Hamodrakas, Stavros J %A Hansson, Bill S %A Huguet, Elisabeth %A Jermiin, Lars S %A Lan, Que %A Lehman, Herman K %A Lorenzen, Marce %A Merzendorfer, Hans %A Michalopoulos, Ioannis %A Morton, David B %A Muthukrishnan, Subbaratnam %A Oakeshott, John G %A Palmer, Will %A Park, Yoonseong %A Passarelli, A Lorena %A Rozas, Julio %A Schwartz, Lawrence M %A Smith, Wendy %A Southgate, Agnes %A Vilcinskas, Andreas %A Vogt, Richard %A Wang, Ping %A Werren, John %A Yu, Xiao-Qiang %A Zhou, Jing-Jiang %A Brown, Susan J %A Scherer, Steven E %A Richards, Stephen %A Blissard, Gary W %K Animals %K Gene Expression %K Gene Expression Profiling %K Genome, Insect %K Larva %K Manduca %K Pupa %K Sequence Analysis, DNA %K Synteny %X

Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.

%B Insect Biochem Mol Biol %V 76 %P 118-147 %8 2016 Sep %G eng %1 https://www.ncbi.nlm.nih.gov/pubmed/27522922?dopt=Abstract %R 10.1016/j.ibmb.2016.07.005 %0 Journal Article %J Nature %D 2008 %T The genome of the model beetle and pest Tribolium castaneum. %A Stephen Richards %A Richard A Gibbs %A Weinstock, George M %A Brown, Susan J %A Denell, Robin %A Beeman, Richard W %A Richard A Gibbs %A Beeman, Richard W %A Brown, Susan J %A Bucher, Gregor %A Friedrich, Markus %A Grimmelikhuijzen, Cornelis J P %A Klingler, Martin %A Lorenzen, Marce %A Stephen Richards %A Roth, Siegfried %A Schröder, Reinhard %A Tautz, Diethard %A Zdobnov, Evgeny M %A Donna M Muzny %A Richard A Gibbs %A Weinstock, George M %A Attaway, Tony %A Bell, Stephanie %A Buhay, Christian J %A Chandrabose, Mimi N %A Chavez, Dean %A Clerk-Blankenburg, Kerstin P %A Cree, Andrew %A Dao, Marvin %A Davis, Clay %A Chacko, Joseph %A Dinh, Huyen %A Dugan-Rocha, Shannon %A Fowler, Gerald %A Garner, Toni T %A Garnes, Jeffrey %A Gnirke, Andreas %A Hawes, Alica %A Hernandez, Judith %A Hines, Sandra %A Holder, Michael %A Hume, Jennifer %A Jhangiani, Shalini N %A Joshi, Vandita %A Ziad Khan %A Jackson, Laronda %A Kovar, Christie %A Kowis, Andrea %A Lee, Sandra %A Lewis, Lora R %A Margolis, Jon %A Morgan, Margaret %A Nazareth, Lynne V %A Nguyen, Ngoc %A Okwuonu, Geoffrey %A Parker, David %A Stephen Richards %A Ruiz, San-Juana %A Santibanez, Jireh %A Savard, Joël %A Steven E Scherer %A Schneider, Brian %A Sodergren, Erica %A Tautz, Diethard %A Vattahil, Selina %A Villasana, Donna %A White, Courtney S %A Wright, Rita %A Park, Yoonseong %A Beeman, Richard W %A Lord, Jeff %A Oppert, Brenda %A Lorenzen, Marce %A Brown, Susan %A Wang, Liangjiang %A Savard, Joël %A Tautz, Diethard %A Stephen Richards %A Weinstock, George %A Richard A Gibbs %A Liu, Yue %A Kim C Worley %A Weinstock, George %A Elsik, Christine G %A Reese, Justin T %A Elhaik, Eran %A Landan, Giddy %A Graur, Dan %A Arensburger, Peter %A Atkinson, Peter %A Beeman, Richard W %A Beidler, Jim %A Brown, Susan J %A Demuth, Jeffery P %A Drury, Douglas W %A Du, Yu-Zhou %A Fujiwara, Haruhiko %A Lorenzen, Marce %A Maselli, Vincenza %A Osanai, Mizuko %A Park, Yoonseong %A Robertson, Hugh M %A Tu, Zhijian %A Wang, Jian-jun %A Wang, Suzhi %A Stephen Richards %A Song, Henry %A Zhang, Lan %A Sodergren, Erica %A Werner, Doreen %A Stanke, Mario %A Morgenstern, Burkhard %A Solovyev, Victor %A Kosarev, Peter %A Brown, Garth %A Chen, Hsiu-Chuan %A Ermolaeva, Olga %A Hlavina, Wratko %A Kapustin, Yuri %A Kiryutin, Boris %A Kitts, Paul %A Maglott, Donna %A Pruitt, Kim %A Sapojnikov, Victor %A Souvorov, Alexandre %A Mackey, Aaron J %A Waterhouse, Robert M %A Wyder, Stefan %A Zdobnov, Evgeny M %A Zdobnov, Evgeny M %A Wyder, Stefan %A Kriventseva, Evgenia V %A Kadowaki, Tatsuhiko %A Bork, Peer %A Aranda, Manuel %A Bao, Riyue %A Beermann, Anke %A Berns, Nicola %A Bolognesi, Renata %A Bonneton, François %A Bopp, Daniel %A Brown, Susan J %A Bucher, Gregor %A Butts, Thomas %A Chaumot, Arnaud %A Denell, Robin E %A Ferrier, David E K %A Friedrich, Markus %A Gordon, Cassondra M %A Jindra, Marek %A Klingler, Martin %A Lan, Que %A Lattorff, H Michael G %A Laudet, Vincent %A von Levetsow, Cornelia %A Liu, Zhenyi %A Lutz, Rebekka %A Lynch, Jeremy A %A da Fonseca, Rodrigo Nunes %A Posnien, Nico %A Reuter, Rolf %A Roth, Siegfried %A Savard, Joël %A Schinko, Johannes B %A Schmitt, Christian %A Schoppmeier, Michael %A Schröder, Reinhard %A Shippy, Teresa D %A Simonnet, Franck %A Marques-Souza, Henrique %A Tautz, Diethard %A Tomoyasu, Yoshinori %A Trauner, Jochen %A Van der Zee, Maurijn %A Vervoort, Michel %A Wittkopp, Nadine %A Wimmer, Ernst A %A Yang, Xiaoyun %A Jones, Andrew K %A Sattelle, David B %A Ebert, Paul R %A Nelson, David %A Scott, Jeffrey G %A Beeman, Richard W %A Muthukrishnan, Subbaratnam %A Kramer, Karl J %A Arakane, Yasuyuki %A Beeman, Richard W %A Zhu, Qingsong %A Hogenkamp, David %A Dixit, Radhika %A Oppert, Brenda %A Jiang, Haobo %A Zou, Zhen %A Marshall, Jeremy %A Elpidina, Elena %A Vinokurov, Konstantin %A Oppert, Cris %A Zou, Zhen %A Evans, Jay %A Lu, Zhiqiang %A Zhao, Picheng %A Sumathipala, Niranji %A Altincicek, Boran %A Vilcinskas, Andreas %A Williams, Michael %A Hultmark, Dan %A Hetru, Charles %A Jiang, Haobo %A Grimmelikhuijzen, Cornelis J P %A Hauser, Frank %A Cazzamali, Giuseppe %A Williamson, Michael %A Park, Yoonseong %A Li, Bin %A Tanaka, Yoshiaki %A Predel, Reinhard %A Neupert, Susanne %A Schachtner, Joachim %A Verleyen, Peter %A Raible, Florian %A Bork, Peer %A Friedrich, Markus %A Walden, Kimberly K O %A Robertson, Hugh M %A Angeli, Sergio %A Forêt, Sylvain %A Bucher, Gregor %A Schuetz, Stefan %A Maleszka, Ryszard %A Wimmer, Ernst A %A Beeman, Richard W %A Lorenzen, Marce %A Tomoyasu, Yoshinori %A Miller, Sherry C %A Grossmann, Daniela %A Bucher, Gregor %K Animals %K Base Composition %K Body Patterning %K Cytochrome P-450 Enzyme System %K DNA Transposable Elements %K Genes, Insect %K Genome, Insect %K Growth and Development %K Humans %K Insecticides %K Neurotransmitter Agents %K Oogenesis %K Phylogeny %K Proteome %K Receptors, G-Protein-Coupled %K Receptors, Odorant %K Repetitive Sequences, Nucleic Acid %K RNA Interference %K Taste %K Telomere %K Tribolium %K Vision, Ocular %X

Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model organism for the study of generalized insect development, and an important pest of stored agricultural products. We describe its genome sequence here. This omnivorous beetle has evolved the ability to interact with a diverse chemical environment, as shown by large expansions in odorant and gustatory receptors, as well as P450 and other detoxification enzymes. Development in Tribolium is more representative of other insects than is Drosophila, a fact reflected in gene content and function. For example, Tribolium has retained more ancestral genes involved in cell-cell communication than Drosophila, some being expressed in the growth zone crucial for axial elongation in short-germ development. Systemic RNA interference in T. castaneum functions differently from that in Caenorhabditis elegans, but nevertheless offers similar power for the elucidation of gene function and identification of targets for selective insect control.

%B Nature %V 452 %P 949-55 %8 2008 Apr 24 %G eng %N 7190 %1 https://www.ncbi.nlm.nih.gov/pubmed/18362917?dopt=Abstract %R 10.1038/nature06784