Expression of the platelet-derived growth factor beta receptor during organogenesis and tissue differentiation in the mouse embryo.

TitleExpression of the platelet-derived growth factor beta receptor during organogenesis and tissue differentiation in the mouse embryo.
Publication TypeJournal Article
Year of Publication1994
AuthorsShinbrot, E, Peters, KG, Williams, LT
JournalDev Dyn
Volume199
Issue3
Pagination169-75
Date Published1994 Mar
ISSN1058-8388
KeywordsAnimals, Blood Vessels, Bone and Bones, Bone Development, Cell Differentiation, DNA, Embryo, Mammalian, Embryonic and Fetal Development, Immunohistochemistry, In Situ Hybridization, Mesoderm, Mice, Myocardium, Receptors, Platelet-Derived Growth Factor, RNA, Messenger
Abstract

In this study we used in situ hybridization to localize expression of the platelet-derived growth factor beta (PDGF beta) receptor mRNA during organogenesis in the mouse embryo (E 9.5-16.5). Expression was first seen in periaortic mesenchyme (E 9.5-10.5). Later (E 12.5-E 16.5), the receptor was expressed in the mesenchymal component of many developing tissues and organs, particularly derivatives of the primitive gut. The expression was exceptionally high in mesenchyme directly supporting an epithelium, typical of many developing organs such as the trachea and intestine. However, as the mesenchyme differentiated into smooth muscle, PDGF beta receptor mRNA was no longer detected. The expression of the PDGF beta receptor mRNA in mesenchymal components of developing organs, along with its absence in epithelial tissues, indicates that it may play a role in mesenchymal-epithelial interactions during organ development. Somewhat unexpectedly, the PDGF beta receptor was highly expressed in the endothelium of small blood vessels and vascular structures such as the hyaloid plexus and choroid plexus. In large blood vessels, PDGF beta receptor mRNA was found in the mesenchyme surrounding the endothelium. This suggests that the PDGF beta receptor is involved in growth and development of blood vessels.

DOI10.1002/aja.1001990302
Alternate JournalDev. Dyn.
PubMed ID8018985