Expression profiling using human tissues in combination with RNA amplification and microarray analysis: assessment of Langerhans cell histiocytosis.

TitleExpression profiling using human tissues in combination with RNA amplification and microarray analysis: assessment of Langerhans cell histiocytosis.
Publication TypeJournal Article
Year of Publication2005
AuthorsMcClain, KL, Cai, Y-H, Hicks, J, Peterson, LE, Yan, X-T, Che, S, Ginsberg, SD
JournalAmino Acids
Volume28
Issue3
Pagination279-90
Date Published2005 May
ISSN0939-4451
KeywordsGene Expression Profiling, Gene Expression Regulation, Histiocytosis, Langerhans-Cell, Humans, Nucleic Acid Amplification Techniques, Oligonucleotide Array Sequence Analysis
Abstract

Advances in molecular genetics have led to sequencing of the human genome, and expression data is becoming available for many diverse tissues throughout the body, allowing for exciting hypothesis testing of critical concepts such as development, differentiation, homeostasis, and ultimately, disease pathogenesis. At present, an optimal methodology to assess gene expression is to evaluate single cells, either identified physiologically in living preparations, or by immunocytochemical or histochemical procedures in fixed cells in vitro or in vivo. Unfortunately, the quantity of RNA harvested from a single cell is not sufficient for standard RNA extraction methods. Therefore, exponential polymerase-chain reaction (PCR) based analyses, and linear RNA amplification including amplified antisense (aRNA) RNA amplification and a newly developed terminal continuation (TC) RNA amplification methodology have been used in combination with microdissection procedures such as laser capture microdissection (LCM) to enable the use of microarray platforms within individual populations of cells obtained from a variety of human tissue sources such as biopsy-derived samples {including Langerhans cell histiocytosis (LCH)} as well as postmortem brain samples for high throughput expression profiling and related downstream genetic analyses.

DOI10.1007/s00726-005-0177-x
Alternate JournalAmino Acids
PubMed ID15791395
Grant ListAG10668 / AG / NIA NIH HHS / United States
AG14449 / AG / NIA NIH HHS / United States
CA94520 / CA / NCI NIH HHS / United States
NS43939 / NS / NINDS NIH HHS / United States