A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13.

TitleA gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13.
Publication TypeJournal Article
Year of Publication1996
AuthorsAnsari-Lari, MA, Muzny, DM, Lu, J, Lu, F, Lilley, CE, Spanos, S, Malley, T, Gibbs, RA
JournalGenome Res
Volume6
Issue4
Pagination314-26
Date Published1996 Apr
ISSN1088-9051
KeywordsAlternative Splicing, Amino Acid Sequence, Base Sequence, CD4 Antigens, Chromosome Mapping, Chromosomes, Human, Pair 12, DNA, Complementary, Humans, Molecular Sequence Data, Multigene Family, Sequence Homology, Amino Acid, Triose-Phosphate Isomerase
Abstract

The genomic sequence of the human CD4 gene and its neighboring region, located at chromosome 12p13, was generated using the large-scale shotgun sequencing strategy. A total of 117 kb of genomic sequence and approximately 11 kb of cDNA sequence were obtained. Six genes, including CD4, triosephosphate isomerase, B3 subunit of G proteins (GNB3), and ubiquitin isopeptidase T (ISOT), with known functions, and two new genes with unknown functions were identified. Using a battery of strategies, the exon/intron boundaries, splice variants, and tissue expression patterns of the genes were determined. Various computer software was utilized for analyses of the DNA and amino acid sequences. The results of the analyses and sequence-based strategies for gene identification are discussed.

Alternate JournalGenome Res.
PubMed ID8723724
Grant ListP30 HG00210 / HG / NHGRI NIH HHS / United States
R0I HG00823 / HG / NHGRI NIH HHS / United States