Genomic imprinting effects on adult body composition in mice.

TitleGenomic imprinting effects on adult body composition in mice.
Publication TypeJournal Article
Year of Publication2008
AuthorsCheverud, JM, Hager, R, Roseman, C, Fawcett, G, Wang, B, Wolf, JB
JournalProc Natl Acad Sci U S A
Volume105
Issue11
Pagination4253-8
Date Published2008 Mar 18
ISSN1091-6490
KeywordsAging, Animals, Body Composition, Body Weight, Female, Genomic Imprinting, Male, Mice, Quantitative Trait Loci
Abstract

Genomic imprinting results in the differential expression of genes, depending on which allele is inherited from the mother and which from the father. The effects of such differential gene expression are reflected in phenotypic differences between the reciprocal heterozygotes (Aa vs. aA). Although many imprinted genes have been identified and play a key role in development, little is known about the contribution of imprinting to quantitative variation in trait expression. Here, we examine this problem by mapping imprinting effects on adult body composition traits in the F(3) generation of an intercross between the Large (LG/J) and Small (SM/J) inbred mouse strains. We identified eight pleiotropic imprinted quantitative trait loci (iQTL) located throughout the genome. Most iQTL are in novel locations that have not previously been associated with imprinting effects, but those on chromosomes 7, 12, and centromeric 18 lie in regions previously identified as containing imprinted genes. Our results show that the effects of genomic imprinting are relatively small, with reciprocal heterozygotes differing by approximately 0.25 standard deviation units and the effects at each locus accounting for 1% to 4% of the phenotypic variance. We detected a variety of imprinting patterns, with paternal expression being the most common. These results indicate that genomic imprinting has small, but detectable, effects on the normal variation of complex traits in adults and is likely to be more common than usually thought.

DOI10.1073/pnas.0706562105
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID18337500
PubMed Central IDPMC2393747
Grant List / / Biotechnology and Biological Sciences Research Council / United Kingdom
DK055736 / DK / NIDDK NIH HHS / United States
BB/C516936/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
R01 DK055736 / DK / NIDDK NIH HHS / United States
R56 DK055736 / DK / NIDDK NIH HHS / United States