Genomic innovations, transcriptional plasticity and gene loss underlying the evolution and divergence of two highly polyphagous and invasive Helicoverpa pest species.

TitleGenomic innovations, transcriptional plasticity and gene loss underlying the evolution and divergence of two highly polyphagous and invasive Helicoverpa pest species.
Publication TypeJournal Article
Year of Publication2017
AuthorsPearce, SL, Clarke, DF, East, PD, Elfekih, S, Gordon, KHJ, Jermiin, LS, McGaughran, A, Oakeshott, JG, Papanikolaou, A, Perera, OP, Rane, RV, Richards, S, Tay, WT, Walsh, TK, Anderson, A, Anderson, CJ, Asgari, S, Board, PG, Bretschneider, A, Campbell, PM, Chertemps, T, Christeller, JT, Coppin, CW, Downes, SJ, Duan, G, Farnsworth, CA, Good, RT, Han, LB, Han, YC, Hatje, K, Horne, I, Huang, YP, Hughes, DST, Jacquin-Joly, E, James, W, Jhangiani, S, Kollmar, M, Kuwar, SS, Li, S, Liu, N-Y, Maibeche, MT, Miller, JR, Montagne, N, Perry, T, Qu, J, Song, SV, Sutton, GG, Vogel, H, Walenz, BP, Xu, W, Zhang, H-J, Zou, Z, Batterham, P, Edwards, OR, Feyereisen, R, Gibbs, RA, Heckel, DG, McGrath, A, Robin, C, Scherer, SE, Worley, KC, Wu, YD
JournalBMC Biol
Volume15
Issue1
Pagination63
Date Published2017 Jul 31
ISSN1741-7007
Abstract

BACKGROUND: Helicoverpa armigera and Helicoverpa zea are major caterpillar pests of Old and New World agriculture, respectively. Both, particularly H. armigera, are extremely polyphagous, and H. armigera has developed resistance to many insecticides. Here we use comparative genomics, transcriptomics and resequencing to elucidate the genetic basis for their properties as pests.

RESULTS: We find that, prior to their divergence about 1.5 Mya, the H. armigera/H. zea lineage had accumulated up to more than 100 more members of specific detoxification and digestion gene families and more than 100 extra gustatory receptor genes, compared to other lepidopterans with narrower host ranges. The two genomes remain very similar in gene content and order, but H. armigera is more polymorphic overall, and H. zea has lost several detoxification genes, as well as about 50 gustatory receptor genes. It also lacks certain genes and alleles conferring insecticide resistance found in H. armigera. Non-synonymous sites in the expanded gene families above are rapidly diverging, both between paralogues and between orthologues in the two species. Whole genome transcriptomic analyses of H. armigera larvae show widely divergent responses to different host plants, including responses among many of the duplicated detoxification and digestion genes.

CONCLUSIONS: The extreme polyphagy of the two heliothines is associated with extensive amplification and neofunctionalisation of genes involved in host finding and use, coupled with versatile transcriptional responses on different hosts. H. armigera's invasion of the Americas in recent years means that hybridisation could generate populations that are both locally adapted and insecticide resistant.

DOI10.1186/s12915-017-0402-6
Alternate JournalBMC Biol.
PubMed ID28756777
PubMed Central IDPMC5535293
Grant ListR01 GM077117 / GM / NIGMS NIH HHS / United States