The genomic organization of Isopeptidase T-3 (ISOT-3), a new member of the ubiquitin specific protease family (UBP).

TitleThe genomic organization of Isopeptidase T-3 (ISOT-3), a new member of the ubiquitin specific protease family (UBP).
Publication TypeJournal Article
Year of Publication1998
AuthorsTimms, KM, Ansari-Lari, MA, Morris, W, Brown, SN, Gibbs, RA
Date Published1998 Sep 14
KeywordsAmino Acid Sequence, Animals, Base Sequence, Carbon-Nitrogen Lyases, Chromosome Mapping, Chromosomes, Human, Pair 3, Consensus Sequence, DNA, Complementary, Exons, Female, Gene Library, Humans, Male, Mice, Molecular Sequence Data, Organ Specificity, Ovary, Polymerase Chain Reaction, Recombinant Proteins, Saccharomyces cerevisiae, Sequence Alignment, Sequence Homology, Amino Acid, Substrate Specificity, Testis, Ubiquitins

A novel Isopeptidase T gene (ISOT-3) has been identified on human mosome 3q26.2--q26.3. gene shows 67.3% nucleotide identity and 54.8% amino acid identity to n Isopeptidase (ISOT-1). Northern blot analysis has shown that ISOT-3 is highly essed in ovary and testes, low-level expression in six other tissues tested. In contrast, ISOT-1 is essed at high levels in brain, and there is no detectable expression in ovary. The exonic nization of these two genes highly conserved with only one variant intron position. Intron 15 in -3 is absent in ISOT-1, there is an alternate splice site at the same location. Although the --intron structure has been erved between the two genes, ISOT-3 has significantly larger intronic ons, and the overall of this gene is at least 90 kb compared to 15 kb for ISOT-1. These data suggest that both ISOT-1 and ISOT-3 have descended from a common ancestor. In addition, the low overall sequence identity and different expression patterns may reflect differences in substrate specificity.

Alternate JournalGene
PubMed ID9841226
Grant ListP30 HG00210 / HG / NHGRI NIH HHS / United States
R01 HG00823 / HG / NHGRI NIH HHS / United States