Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene.

TitleGenomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene.
Publication TypeJournal Article
Year of Publication1993
AuthorsGuo, W, Worley, KC, Adams, V, Mason, J, Sylvester-Jackson, D, Zhang, YH, Towbin, JA, Fogt, DD, Madu, S, Wheeler, DA
JournalNat Genet
Volume4
Issue4
Pagination367-72
Date Published1993 Aug
ISSN1061-4036
KeywordsAmino Acid Sequence, Bacillus subtilis, Base Sequence, Chromosome Mapping, Cloning, Molecular, Cosmids, Escherichia coli, Gene Library, Glycerol Kinase, Humans, Liver, Molecular Sequence Data, Oligodeoxyribonucleotides, Polymerase Chain Reaction, Sequence Homology, Amino Acid, X Chromosome
Abstract

Rapid genomic scanning methods are required to identify expressed sequences and we report an efficient, sensitive and specific approach which relies upon hybridization of an amplified, labeled cDNA library to digested cosmid DNA. We identified expressed sequences within a cosmid in the glycerol kinase (GK) "critical region" of Xp21 that had impressive similarity to prokaryotic GKs. We used this genomic sequence information to clone the human hepatic GK cDNA. Independent confirmation of the identity of this gene was obtained by functional complementation of GK deficient E. coli mutants with a construct containing the complete human X-linked GK coding sequence.

DOI10.1038/ng0893-367
Alternate JournalNat. Genet.
PubMed ID8401584
Grant List5 P30 HD24064 / HD / NICHD NIH HHS / United States
5 P30 HD27823 / HD / NICHD NIH HHS / United States
5 R01 HD22563 / HD / NICHD NIH HHS / United States