|Title||Identification of a single-stranded DNA virus associated with citrus chlorotic dwarf disease, a new member in the family Geminiviridae.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Loconsole, G, Saldarelli, P, Doddapaneni, HV, Savino, V, Martelli, GP, Saponari, M|
|Date Published||2012 Oct 10|
|Keywords||Citrus, DNA, Single-Stranded, DNA, Viral, Geminiviridae, Genome, Viral, Molecular Sequence Data, Open Reading Frames, Plant Diseases, Sequence Analysis, DNA|
In the attempt to identify the causal agent of Citrus chlorotic dwarf disease (CCDD), a virus-like disorder of citrus, the small RNA fraction and total DNA from symptomatic citrus plants were subjected to high-throughput sequencing. DNA fragments deriving from an apparently new geminivirus-like agent were found and assembled by NGS to re-construct the entire viral genome. The newly identified virus has a circular single-stranded DNA genome comprising five open reading frames (ORFs) with sequence homologies with those encoded by geminiviruses. PCR and qPCR assays were successfully used for determining its presence in the CCDD-affected plants obtained by graft propagation. The larger genome size (3.64 vs. 2.5-3.0 kb) and a number of differences in its structural organization, identified this virus as a highly divergent member of the family Geminiviridae, to which the provisional name of Citrus chlorotic dwarf-associated virus (CCDaV) is assigned.