Leptin mediates tumor-stromal interactions that promote the invasive growth of breast cancer cells.

TitleLeptin mediates tumor-stromal interactions that promote the invasive growth of breast cancer cells.
Publication TypeJournal Article
Year of Publication2012
AuthorsBarone, I, Catalano, S, Gelsomino, L, Marsico, S, Giordano, C, Panza, S, Bonofiglio, D, Bossi, G, Covington, KR, Fuqua, SAW, Andò, S
JournalCancer Res
Volume72
Issue6
Pagination1416-27
Date Published2012 Mar 15
ISSN1538-7445
KeywordsBreast Neoplasms, Carcinoma, Cell Line, Tumor, Cell Movement, Cell Proliferation, Epidermal Growth Factor, Estrogen Receptor alpha, Female, Fibroblasts, Gene Expression Profiling, Humans, Leptin, Neoplasm Invasiveness, Receptors, Leptin, Signal Transduction, Stromal Cells
Abstract

Obesity confers risks to cancer development and progression but the mechanisms underlying these risks remain unclear. In this study, we identify a role for the obesity cytokine leptin, which has been implicated previously in breast cancer development, as a determinant for the tumor-promoting activity of cancer-associated fibroblasts (CAF) in both wild-type (WT) and K303R mutant estrogen receptor-α (ERα)-expressing breast cancer cells. Human CAFs stimulated a greater increase in the proliferation and migration of breast cancer cells expressing the K303R-ERα hyperactive receptor than WT-ERα-expressing cells. A concomitant increase was seen in leptin receptor isoform expression and activation of the leptin signaling pathway in cells expressing K303R-ERα compared with WT-ERα, correlating with leptin effects on cell growth, motility, and invasiveness in mutant cells. Epidermal growth factor and other factors secreted by K303R-ERα cells stimulated CAF proliferation, migration, and subsequent leptin secretion. Moreover, K303R-ERα expression generated a leptin hypersensitive phenotype in vivo. Together, our results reveal a bidirectional cross-talk between breast cancer cells and "educated" CAFs that drives tumor progression via leptin signaling. In elucidating a mechanism that connects obesity and cancer, these findings reinforce the concept that blocking cancer-stromal cell communication may represent an effective strategy for targeted therapy of breast cancer.

DOI10.1158/0008-5472.CAN-11-2558
Alternate JournalCancer Res.
PubMed ID22282662
PubMed Central IDPMC4123759
Grant ListR01 CA072038 / CA / NCI NIH HHS / United States
R01 CA72038 / CA / NCI NIH HHS / United States