Linkage mapping in Papio baboons: conservation of a syntenic group of six markers on human chromosome 1.

TitleLinkage mapping in Papio baboons: conservation of a syntenic group of six markers on human chromosome 1.
Publication TypeJournal Article
Year of Publication1995
AuthorsRogers, J, Witte, SM, Kammerer, CM, Hixson, JE, MacCluer, JW
JournalGenomics
Volume28
Issue2
Pagination251-4
Date Published1995 Jul 20
ISSN0888-7543
KeywordsAnimals, Antithrombin III, Chromosome Mapping, Evolution, Molecular, Female, Genes, Genetic Linkage, Genetic Markers, Humans, Male, Microsatellite Repeats, Papio, Polymorphism, Restriction Fragment Length, Recombination, Genetic, Species Specificity
Abstract

We have established multipoint genetic linkage among six loci in baboons (Papio hamadryas). Published PCR primers designed to amplify five human microsatellite loci were used to amplify homologous loci in 229 pedigreed baboons. Southern blotting was used to type two RFLPs in a functional gene (anti-thrombin III) in a subset of those animals. All six loci are known to map to human chromosome 1q, a region of the genome predicted by karyotype studies to be conserved in baboons. Pairwise recombination frequencies and lod scores indicate that the six loci are also linked in baboons. Recombination distances among the loci are similar to those reported for humans. Like humans, the baboons exhibit higher rates of recombination in females than in males. This study demonstrates that (1) microsatellite loci first described and characterized in the human genome can be effectively used for genetic linkage mapping in non-human primates, (2) a group of genetic loci known to be linked on human chromosome 1q are also linked in the baboon genome, and (3) sex differences in recombination frequencies among loci on human chromosome 1q are also observed in the genome of this Old World monkey. This constitutes the first reported multipoint linkage map in any nonhuman primate.

DOI10.1006/geno.1995.1138
Alternate JournalGenomics
PubMed ID8530033
Grant ListHG00336 / HG / NHGRI NIH HHS / United States
HV53030 / HV / NHLBI NIH HHS / United States
RR08781 / RR / NCRR NIH HHS / United States