Molecular characterization of 125I decay and X-ray-induced HPRT mutants in CHO cells.

TitleMolecular characterization of 125I decay and X-ray-induced HPRT mutants in CHO cells.
Publication TypeJournal Article
Year of Publication1987
AuthorsGibbs, RA, Camakaris, J, Hodgson, GS, Martin, RF
JournalInt J Radiat Biol Relat Stud Phys Chem Med
Volume51
Issue2
Pagination193-9
Date Published1987 Feb
ISSN0020-7616
KeywordsAnimals, Chromosome Deletion, Cricetinae, DNA Repair, Gamma Rays, Hypoxanthine Phosphoribosyltransferase, Iodine Radioisotopes, Mutation, Polymorphism, Restriction Fragment Length, X-Rays
Abstract

The predominant initial lesion induced by 125I decay in DNa is a double-strand DNA break comprising a 'mini deletion' of up to several base pairs (bp). However, Southern analysis of the structure of 125I decay-induced HPRT mutants in Chinese hamster ovary cells revealed that most of the mutants are deletions of thousands of bps. A similar predominance of substantial deletions was also found in X-ray-induced mutants.

Alternate JournalInt. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med.
PubMed ID2881903