Molecular cloning of the wild-type and mutant thyA gene from Shigella flexneri Y.

TitleMolecular cloning of the wild-type and mutant thyA gene from Shigella flexneri Y.
Publication TypeJournal Article
Year of Publication1994
AuthorsNur-E-Kamal, MS, Mamun, AA al, Ahmed, ZU
JournalMicrobiol Immunol
Volume38
Issue4
Pagination309-12
Date Published1994
ISSN0385-5600
KeywordsBase Sequence, Cloning, Molecular, Escherichia coli, Genes, Bacterial, Molecular Sequence Data, Mutation, Phenotype, Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Shigella flexneri, Thymidylate Synthase
Abstract

The thyA gene which codes for thymidylate synthase has been cloned and sequenced from the wild-type Shigella flexneri Y strain SH4 and a thyA mutant TSF21 after amplifying the gene by polymerase chain reaction (PCR). The nucleotide sequence revealed 98% homology to the E. coli K-12 thyA gene. The sequence of the wild-type thyA gene of Shigella flexneri Y was identical with that of the thyA mutant except that the residue T at position 345 was replaced by residue A in the thyA mutant. This change would cause a predicted amino acid substitution of leucine at position 44 in the polypeptide product of the wild type by glutamine in the mutant. Thus, Leu44 may be critical in enzymatic activity of the thyA gene product thymidylate synthase.

Alternate JournalMicrobiol. Immunol.
PubMed ID7935051