Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta.

TitleMultifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta.
Publication TypeJournal Article
Year of Publication2016
AuthorsKanost, MR, Arrese, EL, Cao, X, Chen, Y-R, Chellapilla, S, Goldsmith, MR, Grosse-Wilde, E, Heckel, DG, Herndon, N, Jiang, H, Papanicolaou, A, Qu, J, Soulages, JL, Vogel, H, Walters, J, Waterhouse, RM, Ahn, S-J, Almeida, FC, An, C, Aqrawi, P, Bretschneider, A, Bryant, WB, Bucks, S, Chao, H, Chevignon, G, Christen, JM, Clarke, DF, Dittmer, NT, Ferguson, LCF, Garavelou, S, Gordon, KHJ, Gunaratna, RT, Han, Y, Hauser, F, He, Y, Heidel-Fischer, H, Hirsh, A, Hu, Y, Jiang, H, Kalra, D, Klinner, C, König, C, Kovar, C, Kroll, AR, Kuwar, SS, Lee, SL, Lehman, R, Li, K, Li, Z, Liang, H, Lovelace, S, Lu, Z, Mansfield, JH, McCulloch, KJ, Mathew, T, Morton, B, Muzny, DM, Neunemann, D, Ongeri, F, Pauchet, Y, Pu, L-L, Pyrousis, I, Rao, X-J, Redding, A, Roesel, C, Sánchez-Gracia, A, Schaack, S, Shukla, A, Tetreau, G, Wang, Y, Xiong, G-H, Traut, W, Walsh, TK, Worley, KC, Wu, D, Wu, W, Wu, Y-Q, Zhang, X, Zou, Z, Zucker, H, Briscoe, AD, Burmester, T, Clem, RJ, Feyereisen, R, Grimmelikhuijzen, CJP, Hamodrakas, SJ, Hansson, BS, Huguet, E, Jermiin, LS, Lan, Q, Lehman, HK, Lorenzen, M, Merzendorfer, H, Michalopoulos, I, Morton, DB, Muthukrishnan, S, Oakeshott, JG, Palmer, W, Park, Y, A Passarelli, L, Rozas, J, Schwartz, LM, Smith, W, Southgate, A, Vilcinskas, A, Vogt, R, Wang, P, Werren, J, Yu, X-Q, Zhou, J-J, Brown, SJ, Scherer, SE, Richards, S, Blissard, GW
JournalInsect Biochem Mol Biol
Date Published2016 Sep
KeywordsAnimals, Gene Expression, Gene Expression Profiling, Genome, Insect, Larva, Manduca, Pupa, Sequence Analysis, DNA, Synteny

Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.

Alternate JournalInsect Biochem Mol Biol
PubMed ID27522922
PubMed Central IDPMC5010457
Grant ListR37 GM041247 / GM / NIGMS NIH HHS / United States
F32 AI104154 / AI / NIAID NIH HHS / United States
R01 GM058634 / GM / NIGMS NIH HHS / United States
R01 GM041247 / GM / NIGMS NIH HHS / United States
R01 AI091972 / AI / NIAID NIH HHS / United States
R01 GM064677 / GM / NIGMS NIH HHS / United States

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