Onset of atonal expression in Drosophila retinal progenitors involves redundant and synergistic contributions of Ey/Pax6 and So binding sites within two distant enhancers.

TitleOnset of atonal expression in Drosophila retinal progenitors involves redundant and synergistic contributions of Ey/Pax6 and So binding sites within two distant enhancers.
Publication TypeJournal Article
Year of Publication2014
AuthorsZhou, Q, Zhang, T, Jemc, JC, Chen, Y, Chen, R, Rebay, I, Pignoni, F
JournalDev Biol
Volume386
Issue1
Pagination152-64
Date Published2014 Feb 1
ISSN1095-564X
KeywordsAnimals, Basic Helix-Loop-Helix Transcription Factors, Binding Sites, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Enhancer Elements, Genetic, Eye Proteins, Gene Expression Regulation, Developmental, Green Fluorescent Proteins, Homeodomain Proteins, In Situ Hybridization, Nerve Tissue Proteins, Nervous System, Neurons, Paired Box Transcription Factors, Repressor Proteins, Retina, Stem Cells, Transcription, Genetic
Abstract

Proneural transcription factors drive the generation of specialized neurons during nervous system development, and their dynamic expression pattern is critical to their function. The activation of the proneural gene atonal (ato) in the Drosophila eye disc epithelium represents a critical step in the transition from retinal progenitor cell to developing photoreceptor neuron. We show here that the onset of ato transcription depends on two distant enhancers that function differently in subsets of retinal progenitor cells. A detailed analysis of the crosstalk between these enhancers identifies a critical role for three binding sites for the Retinal Determination factors Eyeless (Ey) and Sine oculis (So). We show how these sites interact to induce ato expression in distinct regions of the eye field and confirm them to be occupied by endogenous Ey and So proteins in vivo. Our study suggests that Ey and So operate differently through the same 3' cis-regulatory sites in distinct populations of retinal progenitors.

DOI10.1016/j.ydbio.2013.11.012
Alternate JournalDev. Biol.
PubMed ID24247006
Grant ListR01 EY013167 / EY / NEI NIH HHS / United States
R01EY013167 / EY / NEI NIH HHS / United States
R01EY016853 / EY / NEI NIH HHS / United States
R01EY12549 / EY / NEI NIH HHS / United States