Title | Onset of atonal expression in Drosophila retinal progenitors involves redundant and synergistic contributions of Ey/Pax6 and So binding sites within two distant enhancers. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Zhou, Q, Zhang, T, Jemc, JC, Chen, Y, Chen, R, Rebay, I, Pignoni, F |
Journal | Dev Biol |
Volume | 386 |
Issue | 1 |
Pagination | 152-64 |
Date Published | 2014 Feb 01 |
ISSN | 1095-564X |
Keywords | Animals, Basic Helix-Loop-Helix Transcription Factors, Binding Sites, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Enhancer Elements, Genetic, Eye Proteins, Gene Expression Regulation, Developmental, Green Fluorescent Proteins, Homeodomain Proteins, In Situ Hybridization, Nerve Tissue Proteins, Nervous System, Neurons, Paired Box Transcription Factors, PAX6 Transcription Factor, Repressor Proteins, Retina, Stem Cells, Transcription, Genetic |
Abstract | Proneural transcription factors drive the generation of specialized neurons during nervous system development, and their dynamic expression pattern is critical to their function. The activation of the proneural gene atonal (ato) in the Drosophila eye disc epithelium represents a critical step in the transition from retinal progenitor cell to developing photoreceptor neuron. We show here that the onset of ato transcription depends on two distant enhancers that function differently in subsets of retinal progenitor cells. A detailed analysis of the crosstalk between these enhancers identifies a critical role for three binding sites for the Retinal Determination factors Eyeless (Ey) and Sine oculis (So). We show how these sites interact to induce ato expression in distinct regions of the eye field and confirm them to be occupied by endogenous Ey and So proteins in vivo. Our study suggests that Ey and So operate differently through the same 3' cis-regulatory sites in distinct populations of retinal progenitors. |
DOI | 10.1016/j.ydbio.2013.11.012 |
Alternate Journal | Dev Biol |
PubMed ID | 24247006 |
PubMed Central ID | PMC5223736 |
Grant List | R01 EY012549 / EY / NEI NIH HHS / United States R01 EY016853 / EY / NEI NIH HHS / United States R01 EY013167 / EY / NEI NIH HHS / United States R01EY013167 / EY / NEI NIH HHS / United States R01EY12549 / EY / NEI NIH HHS / United States R01EY016853 / EY / NEI NIH HHS / United States |
Onset of atonal expression in Drosophila retinal progenitors involves redundant and synergistic contributions of Ey/Pax6 and So binding sites within two distant enhancers.
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