Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032.

TitleParadoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032.
Publication TypeJournal Article
Year of Publication2007
AuthorsGioia, J, Yerrapragada, S, Qin, X, Jiang, H, Igboeli, OC, Muzny, DM, Dugan-Rocha, S, Ding, Y, Hawes, A, Liu, W, Perez, L, Kovar, C, Dinh, H, Lee, S, Nazareth, L, Blyth, P, Holder, M, Buhay, C, Tirumalai, MR, Liu, Y, Dasgupta, I, Bokhetache, L, Fujita, M, Karouia, F, Moorthy, PEswara, Siefert, J, Uzman, A, Buzumbo, P, Verma, A, Zwiya, H, McWilliams, BD, Olowu, A, Clinkenbeard, KD, Newcombe, D, Golebiewski, L, Petrosino, JF, Nicholson, WL, Fox, GE, Venkateswaran, K, Highlander, SK, Weinstock, GM
JournalPLoS One
Volume2
Issue9
Paginatione928
Date Published2007 Sep 26
ISSN1932-6203
KeywordsBacillus, DNA Repair, Drug Resistance, Bacterial, Gamma Rays, Genes, Bacterial, Genome, Bacterial, Hydrogen Peroxide, Oxidative Stress, Sequence Analysis, DNA, Spores, Bacterial, Ultraviolet Rays
Abstract

BACKGROUND: Bacillus spores are notoriously resistant to unfavorable conditions such as UV radiation, gamma-radiation, H2O2, desiccation, chemical disinfection, or starvation. Bacillus pumilus SAFR-032 survives standard decontamination procedures of the Jet Propulsion Lab spacecraft assembly facility, and both spores and vegetative cells of this strain exhibit elevated resistance to UV radiation and H2O2 compared to other Bacillus species.PRINCIPAL FINDINGS: The genome of B. pumilus SAFR-032 was sequenced and annotated. Lists of genes relevant to DNA repair and the oxidative stress response were generated and compared to B. subtilis and B. licheniformis. Differences in conservation of genes, gene order, and protein sequences are highlighted because they potentially explain the extreme resistance phenotype of B. pumilus. The B. pumilus genome includes genes not found in B. subtilis or B. licheniformis and conserved genes with sequence divergence, but paradoxically lacks several genes that function in UV or H2O2 resistance in other Bacillus species.SIGNIFICANCE: This study identifies several candidate genes for further research into UV and H2O2 resistance. These findings will help explain the resistance of B. pumilus and are applicable to understanding sterilization survival strategies of microbes.

DOI10.1371/journal.pone.0000928
Alternate JournalPLoS ONE
PubMed ID17895969
PubMed Central IDPMC1976550