Pooled genomic indexing of rhesus macaque.

TitlePooled genomic indexing of rhesus macaque.
Publication TypeJournal Article
Year of Publication2005
AuthorsMilosavljevic, A, Harris, RA, Sodergren, EJ, Jackson, AR, Kalafus, KJ, Hodgson, A, Cree, A, Dai, W, Csuros, M, Zhu, B, De Jong, PJ, Weinstock, GM, Gibbs, RA
JournalGenome Res
Volume15
Issue2
Pagination292-301
Date Published2005 Feb
ISSN1088-9051
KeywordsAnimals, Chromosome Aberrations, Chromosomes, Artificial, Bacterial, Contig Mapping, DNA, Genetic Markers, Genome, Genome, Human, Humans, Macaca mulatta, Sequence Alignment, Sequence Analysis, DNA
Abstract

Pooled genomic indexing (PGI) is a method for mapping collections of bacterial artificial chromosome (BAC) clones between species by using a combination of clone pooling and DNA sequencing. PGI has been used to map a total of 3858 BAC clones covering approximately 24% of the rhesus macaque (Macaca mulatta) genome onto 4178 homologous loci in the human genome. A number of intrachromosomal rearrangements were detected by mapping multiple segments within the individual rhesus BACs onto multiple disjoined loci in the human genome. Transversal pooling designs involving shuffled BAC arrays were employed for robust mapping even with modest DNA sequence read coverage. A further innovation, short-tag pooled genomic indexing (ST-PGI), was also introduced to further improve the economy of mapping by sequencing multiple, short, mapable tags within a single sequencing reaction.

DOI10.1101/gr.3162505
Alternate JournalGenome Res.
PubMed ID15687293
PubMed Central IDPMC546531
Grant ListR01 HG 02583-01 / HG / NHGRI NIH HHS / United States
R01 HG002583 / HG / NHGRI NIH HHS / United States
U54 HG 02051 / HG / NHGRI NIH HHS / United States
U01 RR 18464 / RR / NCRR NIH HHS / United States
U54 HG002051 / HG / NHGRI NIH HHS / United States
U01 RR018464 / RR / NCRR NIH HHS / United States