Positional cloning of the major quantitative trait locus underlying lung tumor susceptibility in mice.

TitlePositional cloning of the major quantitative trait locus underlying lung tumor susceptibility in mice.
Publication TypeJournal Article
Year of Publication2003
AuthorsZhang, Z, Futamura, M, Vikis, HG, Wang, M, Li, J, Wang, Y, Guan, K-L, You, M
JournalProc Natl Acad Sci U S A
Volume100
Issue22
Pagination12642-7
Date Published2003 Oct 28
ISSN0027-8424
KeywordsAdenoma, Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Chromosome Mapping, Ciona intestinalis, Cloning, Molecular, DNA Primers, Genetic Markers, Genetic Predisposition to Disease, Humans, Lung Neoplasms, Male, Mice, Mice, Inbred A, Mice, Inbred C57BL, Mice, Nude, Microsatellite Repeats, Molecular Sequence Data, Proto-Oncogene Proteins, Proto-Oncogene Proteins p21(ras), Quantitative Trait Loci, ras Proteins, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Homology, Amino Acid
Abstract

Pulmonary adenoma susceptibility 1 (Pas1), located on chromosome 6, is the major locus affecting inherited predisposition to lung tumor development in mice. We have fine mapped the Pas1 locus to a region of approximately 0.5 megabases by using congenic strains of mice, constructed by placing the Pas1 region of chromosome 6 from A/J mice onto the genetic background of C57BL/6J mice. Systematic characterization of Pas1 candidates establishes the Las1 (lung adenoma susceptibility 1) and Kras2 (Kirsten rat sarcoma oncogene 2) genes as primary candidates for the Pas1 locus. Clearly, Kras2 affects lung tumor progression only, and Las1 is likely to affect lung tumor multiplicity.

DOI10.1073/pnas.2133947100
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID14583591
PubMed Central IDPMC240671
Grant ListR01 GM 62694 / GM / NIGMS NIH HHS / United States
R01 GM062694 / GM / NIGMS NIH HHS / United States
R01 CA058554 / CA / NCI NIH HHS / United States
R01 CA 78797 / CA / NCI NIH HHS / United States
R01 CA 58554 / CA / NCI NIH HHS / United States