Potential salmon sperm origin of the E3 region insert of the adenovirus 5 dl309 mutant.

TitlePotential salmon sperm origin of the E3 region insert of the adenovirus 5 dl309 mutant.
Publication TypeJournal Article
Year of Publication1996
AuthorsGingras, M-C, Arevalo, P, Aguilar-Cordova, E
JournalCancer Gene Ther
Volume3
Issue3
Pagination151-4
Date Published1996 May-Jun
ISSN0929-1903
KeywordsAdenovirus E3 Proteins, Adenoviruses, Human, Animals, Avian Sarcoma Viruses, Base Sequence, DNA, DNA Primers, Genetic Vectors, HeLa Cells, Humans, Male, Molecular Sequence Data, Plasmids, Polymerase Chain Reaction, Prolactin, Proton-Translocating ATPases, Repetitive Sequences, Nucleic Acid, Salmon, Sequence Homology, Nucleic Acid, Spermatozoa, Thymidine Kinase, Transfection
Abstract

The plasmid pJM17 is a commonly used adenoviral backbone derived from the dl309 mutant virus. It contains unknown sequences inserted in the E3 region during construction of the dl309 mutant. Complete description of the backbone sequence is required for interpretation of potential vector effects and for regulatory approval of a vector to be used in clinical trials. The anonymous E3 insert was sequenced and analyzed. The insert fragment is 646 base pairs (bp) long and is 100 bp shorter than the vector sequences it replaces. It interrupts the expression of the E3B 10.4K, 14.6K, and 14.7K genes, but not the E3A glycoprotein (gp) 19K gene. Sequence analysis and Southern blotting suggest that the insert might originate from salmon sperm DNA used as carrier during the construction of dl309. Transcription from the insert was not detected by Northern blot analysis of vector-transduced cells but was detected by reverse transcriptase polymerase chain reaction.

Alternate JournalCancer Gene Ther.
PubMed ID8725878
Grant ListHL50422 / HL / NHLBI NIH HHS / United States
P01 HL51754 / HL / NHLBI NIH HHS / United States